Transpiration Report

Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.

This lab is used to test the effect the respiration rates in plants in animals and how it affects the level of carbon dioxide present in the water. Oxygen and carbon dioxide are gases that are vital to all organisms, whether it is given or released through that organism. Both plants and animals use oxygen and carbon dioxide for cellular respiration, giving off carbon dioxide as a waste product. This lab is an example of cellular respiration in both plants and animals. The change in the carbon dioxide levels will be

The independent variable of this experiment are the environmental factors that you are testing on the rate of transpiration, so our independent variables would be bright light, fan, dark, and misted. The dependent variable is the rate of transportation because this depends on whatever environmental factor the plant is placed. The constants were things like the type of plant we used, the room temperature, the type of scale we used and how far the fan/light was placed from the plant.

Introduction: Photosynthesis can be defined as a solar powered process that removes atmospheric carbon dioxide and transforms it into oxygen and carbohydrates (Harris-Haller 2014). Photosynthesis can be considered to be the most important biochemical process on Earth because it helps plants to grow its roots, leaves, and fruits, and plants serve as autotrophs which are crucial to the food chain on earth. Several factors determine the process of photosynthesis. Light is one these factors and is the main subject of this experiment. The intensity of light is a property of light that is important for photosynthesis to occur. Brighter light causes more light to touch the surface of the plant which increases the rate of photosynthesis (Speer 1997). This is why there is a tendency of higher rates of photosynthesis in climates with a lot of sunlight than areas that primarily do not get as much sunlight. Light wavelength is also a property of

The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.

Transpiration is said to be the loss of water vapor through the stomata of the leaves in a plant. Transpiration essentially serves to move water and other nutrients throughout a plant, to cool down plants and humans and to maintain turgor pressure in the cells of plants (sdhydroponics). The transpiration rate in a plant is affected by the wind, light and humidity. temperature and water. The wind serves to determine how dry the air is when transpiration occurs. Light can at times speed up the rate of transpiration in plants. Transpiration tends to occur faster in the light rather than when in the dark. Humidity serves to determine the rate of the diffusion of water in the plant. As

The rate of photosynthesis is affected by environmental factors like light intensity, light wavelength, and temperature. This experiment will test the

There are many procedures during this lab and many materials needed for an accurate analysis of data. First, fill a 100 mL graduated cylinder with 50 mL of water. Add 25 germinating peas and determine the amount of water that is displaced. Record this volume of the 25 germinating peas, then remove the peas and put those peas on a paper towel. They will be used for the first respirometer. Next, refill the graduated cylinder with 50 mL of water and add 25 non-germinating peas to it. Add glass beads to the graduated cylinder until the volume is the same to that of germinating peas. Remove the beads and peas and put on a paper towel. They will be used in respirometer 2. Now, the graduated cylinder was filled once again, determine how many glass beads will be require to reach the same volume of the germinating peas. Remove the beads and they will be used in respirometer 3. Then repeat the procedures used above to prepare a second set of germinating peas, dry peas and beads, and beads to be used in respirometers 4,5,and 6, the only difference is the temperature of the water.

This lab will be driven by the research question, what is the effect of temperature (at 5oC, 15oC, 25oC, 35oC and 45oC) on the photosynthetic rate (measure of oxygen bubbles over a period of time) of Elodea (pondweed)?

The two hypotheses tested during the experiment were the warmer the temperature, the more oxygen will be consumed, and the germinated peas will consume more oxygen than the dry peas. Some conditions that had to remain constant in this experiment were that each vial had to have the same volume to let us know that the volume of the vial wouldn’t affect the respiration rate. Had the volumes been different, the respiration rate might have been affected. Another constant was the 7 minute equilibrium period before completely submerging the vials in the water. This allowed the peas and the other substances in the vials to begin respiration before being completely submerged in the water, but also, they would have the same time to carry out

This lab deals with the transpiration rates in plants, specifically a tomato plant that was used for this experiment. Transpiration is when water leaves a plant through the stomata as water vapor while the stomata is capturing CO2 for photosynthesis. This experiment used three different scenarios: a tomato plant with a light shining on it, a tomato plant with wind blowing on it from a fan, and lastly a tomato plant with nothing acting on it. The hypothesis is that the rate of transpiration will be fastest with light, faster with wind, and slow with the control. This hypothesis was rejected because the rate of transpiration is as follows with the wind having the fastest rate: with light the rate was 7.60 mm/min, with wind 10.20 mm/min, and control 4.33 mm/min. The cause of the wind having a faster transpiration rate than the light may have been due to the surface area of the leaves on the tomato plants. The surface area of the leaves for the wind experiment is 8,124mm2, and for the light is 7,740mm2.By doing this transpiration experiment it helps one to see what happens in plants daily and understand why it happens.

Also, it can be observed that at lower temperatures, ATP cannot be regenerated and in turn effects the production of Ribulose-1,5-bisphosphate (RuBP) (Ensminger et al., 2006). RuBP is used in the first step of the Calvin Cycle to help reduce CO2 (Osborne et al., 2008). Therefore, without RuBP the reactions of the sink will decrease in rate. Perhaps also observed at the molecular level of acclimated and acclimatized plants is the use of isozymes. Osborne et al. (2008) found that isozymes can replace the original enzymes of a plant to work faster and increase the rate of the Calvin Cycle at lower

Purpose of Investigation To study how pH and temperature would effect the reaction rate for amylase on starch. Amylase is an enzyme found in human saliva and pancreas. It is the digestive enzyme that is needed to breakdown starch molecules. Amylase must be kept at certain conditions to function at its optimum level. The efficiency of starch digestion by amylase can be measured by how much simple sugar it produces under various conditions.

However, the photosynthetic process can be affected by different environmental factors. In the following experiment, we tested the effects that the light intensity, light wavelength and pigment had on photosynthesis. The action spectrum of photosynthesis shows which wavelength of light is the most effective using only one line. The absorption spectrum plots how much light is absorbed at different wavelengths by one or more different pigment types. Organisms have different optimal functional ranges, so it is for our benefit to discover the conditions that this process works best. If the environmental conditions of light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will increase with average light intensity and under the wavelengths of white light which will correspond to the absorption spectrum of the pigments. The null hypothesis to this would be; if the environmental conditions light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will decrease with average light intensity and under the white light which will correspond to the absorption spectrum of the pigments.

The Effect of Light Intensity and Temperature on the Rate of Photosynthesis Aim The aim of my experiment is to determine whether intensity of light and temperature would affect the rate of photosynthesis in a plant. To do this, I will place a piece of pondweed in varying light intensities and temperatures, and observe the amount of oxygen being given off. I am using pondweed because of its unusual quality of giving off bubbles of gas from a cut end, when placed in water. Introduction Photosynthesis occurs only in the presence of light, and takes place in the chloroplasts of green plant cells.