Tray Chromatography Lab Report

Data Tables and Observation: Fill out the data sheet (below) for the experiment and submit with this form. Recording data carefully

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

Purpose: The purpose of this lab is to calculate the percent recovery as well as prepare an adsorption chromatography column and finally, separate a mixture using chromatography. Theory: In this lab we will be using chromatography to separate a mixture.

as safety boxes. Currently the company runs two manufacturing facilities, one located in Des Moines, Iowa which manufactures

| The Purpose of this project is to increase overall sales by developing new products, increasing productivity and national presence and reducing dependence on equipment suppliers. To accomplish this creation of a new manufacturing plant is required. The plant will be built in a

The investigation is showing how enzymes work inside a mammal's stomach. Rennin is the enzyme found in young mammals and has more effect

Gather the following lab equipment: Goggles, test tubes, 24 well plate, Gas assembly with copper and plastic tubing and a #00 stopper, short stem pipet, rubber stopper #00 with one hole and a pipet tip with plastic gas delivery tube, 2 small tables of AlkaSeltzer, 4mL Bromothymol blue .04%, 20 mL hydrochloric acid, 4-6 pieces of manganese metal, 4-6 pieces of mossy zinc, and 3 pipet bulbs.

The Canadian fradistat industry consists of four companies: Acme Ltd., Beaver Ltd., Canco Ltd. and Deeco Ltd. Fradistats are components in many industrial products.

The Thin Layer Chromatography of Drugs lab taught me how chromatography could be used to identify the compounds of an unknown drug. This procedure relied on using compounds that had a known travel distance, which served as the control, and examining how far the compounds in the unknown drug traveled. The results of this experiment indicated that drugs are chemical molecules with a diverse amount of compounds i.e.: caffeine, acetaminophen, asprin, etc. The essentials of this lab relied on two factors that allow for the separation of different compounds from the drug.

Procedure: First, we began the lab with the chromatography strip positioned 152 mm tall and 19 mm wide. Using a ruler and pencil, we drew 15 mm from the bottom of the paper across the width. We measured 9.5 mm from the edge and placed a dot with the pencil on the line to mark the starting point. The, we measured 20 mm from the top of the strip and folded across the width of the strip. These few steps were repeated with a second strip.

Chromatography Investigation Chromatography is a highly regarded technique used to separate the components of a mixture. It is based on the principle that each component possesses a unique affinity for a stationary phase and a mobile phase. The components that are more inclined to enter the mobile phase will migrate further on the chromatogram and distinguish themselves from the other components. The type of solvent used in chromatography is known to directly affect the separation of the mixture. In this experiment, thin-layer and column chromatography will be utilized to separate the numerous chlorophyll and carotenoid pigments of a spinach extract.

As shown in figure 1 , schematic diagram of the experimental apparatus is shown. The three pipes used in the fabrication of the heat exchanger are made of brass(inner pipe), copper pipe(helical pipe), mild steel pipe(outer pipe). The inside diameters of the three pipes are 0.03175m, 0.009525m and 0.0635m respectively with thickness of each pipe as 1.5mm only. The experimental set up is composed of a test section, normal water loop, cold water loop, hot water loop and temperature measuring system. The water is used as working fluid. The test section and the connections of piping system are designed such that parts can be changed for different flow arrangement. The hot water loop consists of a storage tank, an electric heater of 3000W immerged inside the storage tank to obtain the high temperature of hot water, a pump and gate valve. The cold water loop consists of a storage tank, a pump and gate valve. Ice cubes are used to cool down the temperature of tap water to nearly 130 C . The normal water loop consists of storage tank, pump and gate valve. After the temperature of the water is adjusted to the desired level, the normal, cold and hot water are pumped out of the storage tank and are passed through test section and discharged to outside. The flow rate of the water are controlled by adjusting the valve and measured by the flow metres of +_ 0.2% of full scale. The heat exchanger is a concentric tube

Gel-Filtration Chromatography is a commonly used method used in order purify a protein from a mixture, by means of separations. Different biomolecules differ in size, or their molecular weight. In the gel matrix inside the chromatography column, there are gel beads which are porous to allow certain sized molecules to enter. The molecules that are able to enter the pores of the gel, are held in stationary phase and will elute from the column later on, these are usually smaller, to medium sized molecules. Larger molecules that are not able to fit in the pores will elute out of the column first, they are involved in mobile phase where they just go straight through the column without interacting with the gel beads. Smaller molecules will have a higher elution volume, while the larger molecules will have a lower elution volume. The volume to elute the protein is inversely proportional to the molecules size.

The experiment is conducted with the help of a Manometer, with which the pressure drop between two gauge points can be measured. For small pressure differences the water (oil) / air manometer is used, whereas for larger pressure differences the mercury / water (oil) manometer is used. The flow rate of the water is measured in volume per time; the flow rate of the oil is measured with a rota meter.

-Ensuring that drain supply is secured and introduction to climate conditions, for example, dry spells are overseen; and