Tributyrin Is A Triacylglycerol

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A practical problem that limits the use of tributyrin, which is a triacylglycerol composed of three butyric acid molecules esterified with glycerol, in in vitro experiments is the fact that tributyrin is highly unstable due to the Ostwald ripening effect, which is a process whereby larger droplets grow at the expense of smaller droplets due to molecular diffusion of oil molecules through the continuous aqueous phase separating the droplets. This may substantially affect results and cause great experiment-to-experiment variations. Based on this consideration, in the in vitro experiments we used sodium butyrate.
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