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Tropb Enzyme Lab Report

Satisfactory Essays
The recent elucidation of the biosynthetic pathway for stipitatic acid led to the identication of a new FMO (TropB).4 This enzyme catalyses the oxidative dearomatisation of 3-methylorcinaldehyde
1 to produce a dienone 2 (Scheme 1A). This hydroxylation step is remarkably similar to one of the steps reported for the chemical synthesis of azaphilones and their analogues (Scheme 1B).5,6
The synthetic step requires extreme cold temperatures (70 to 10 C), hazardous reagents and relatively long reaction time
(30 h). It would be advantageous if related transformations could be performed bio-catalytically using TropB or a related enzyme. This would obviate the utilisation of dangerous chemicals and challenging reaction conditions to facilitate the
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