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Tube 4 Lab Report

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The first hypothesis was unsupported as Tube 4 (positive control) had the most succinate (.2mL) but ended with the lowest % transmittance out of the three sample we were examining (Tubes 2-4). We believe this discrepancy to be due to the variability of the mitochondrial solution. If the Tube 4 mitochondrial suspension had less concentrated enzyme than the solution for Tube 3 and Tube 2 then Tube 4 would be biologically incapable of matching their rate of reduction. It's worth mentioning that Tube 2 and 3 had a %T change of 23.1% and 23.2% respectively while Tube 4 exhibited a change of only 20.4%; further supporting the idea that Tube 4, despite its increase in succinate concentration, wa lacking in other enzymes/proteins necessary for the …show more content…

In the second experiment I knocked over the 24 degrees celsius test tube so the starting volume was 2.5mL in contrast to the other tubes 1 mL initial volume; this exposure may have affected the outcome for this sample. The third experiment was both correct and incorrect as the monosaccharides, fructose and galactose, demonstrated wildly different CO2 production. Sucrose produced about 11mL of CO2 while galactose produced only 0.5mL. The disaccharides of sucrose, maltose, and lactose demonstrated a similar trend where sucrose produced significant amounts of CO2 whole maltose and lactose lagged behind in production. However, fructose still produced significantly more CO2 (1.5mL) than the most productive disaccharide sucrose. This suggest that while the energy barrier of two reactions vs one reaction between monosaccharides and disaccharides might be a contributor, it is more likely that the availability of specialized enzyme is more important. For example, in the data, it is more likely that there is a sucrose enzyme in higher concentration/availability than a galactose enzyme given the substantial difference in CO2

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