Types Of Extreme Temperatures Affect Enzyme Activity

1225 Words Nov 2nd, 2014 5 Pages
Observing Enzyme Catalysis
Purpose:
• Procedure A (Testing Enzyme Activity)- How do different types of extreme temperatures affect enzyme activity?
• Procedure B (Establishing a Baseline)- How much peroxide is needed to establish a baseline for a 1.5% solution?
• Procedure C (Rate of Hydrogen Peroxide Spontaneous Decomposition)- How do oxygen and water affect the spontaneous decomposition of hydrogen peroxide?
• Procedure D (Rate of Hydrogen Peroxide Decomposition by Enzyme Catalysis)-How does different titration techniques affect the rate of hydrogen peroxide decomposition by enzyme catalysis?
Hypothesis:
• Procedure A (Testing Enzyme Activity)- In some conditions, a reaction will occur and in others no reaction will happen.
• Procedure B (Establishing a Baseline)- The amount of KMnO4 added to the solution is proportional to the amount of hydrogen peroxide present in the solution.
• Procedure C (Rate of Hydrogen Peroxide Spontaneous Decomposition)- The amount of hydrogen peroxide left after 24 hours of sitting out at room temperature will be less than the initial amount.
• Procedure D (Rate of Hydrogen Peroxide Decomposition by Enzyme Catalysis)- In the presence of the catalase, hydrogen peroxide (H2O2) will decompose at a faster rate.
Data and Observations: Procedure A was an experiment on observing how different effects of extreme temperatures have on enzyme catalysis. There are three parts in this experiment. In part 1, we took a syringe labeled “H2O2” and used it…
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