Understanding Stress During Puberty

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In this experiment, the author’s focused on understanding stress during puberty and the sex-specific differences of the effects on humans through rat models. As we go through puberty, both male and females have increased levels of sex steroid hormones. In females this causes levels of cortisol to increase, as well as the probability of developing a mood disorder relating to stress. Past studies have shown that this is because of the plasticity of the hypothalamic-pituitary- adrenal axis (HPA) axis and the areas of the brain the regulate mood, such as the amygdala. They supported their previous claim with an experiment that was done on rats where they were subjected to stressful events, known as juvenile social subjugation (JSS), and …show more content…
Autoradiography helps to visualize the CRF1 and CRF2 receptors. The sections of the brain were incubated for 2 hours in a binding chamber that contained 500 mM Astressin 2B and 500mM CP-154,526. The Astressin 2B helps to visualize the CRF1 receptors, while the CP-154,526 helps to visualize the CRF2 receptors. The control group of sections of brains was incubated for 2 hours in 0.2nm 125I Sanguine. They were washed then exposed to Kodak Biomax MR film. Next, they calculated the receptor binding density and background measurements of the lateral dorsal thalamus and the dorsal striatum. They created heat maps of the brain sections, whose densitometry measurements were close to the mean, using Photoshop. They performed two-way ANOVA and post hoc Student’s t-tests. They compared between subject with sex and age, as well as the “effects of age within each sex and sex within each age.”
Results
Overall the Basolateral Nucleus of the Amgdala (BLA) was the primary location for the expression of CRF1 binding and the MePV was the secondary location for binding. CRF2 expression in the MePV was not as intense as that of the CRF1 receptor. The MePD had less expression of both types of CRF receptor and no expression in the CeA. They found intense expression of CRF2 receptor in the cortical nucleus of the amygdala and choroid plexus.
Each hemisphere of the brain had no significant difference in the binding of both types of CRF receptors. Figure 2 shows that in the binding
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