Understanding The Nature Of Bacteria Are The Smallest And Most Versatile Independent Living Organism

To perform this test, a small drop of water is placed on a clean microscope slide. A metal loop that has been properly sterilized in the blue flame and allowed time to cool is used to

1) Apply the stain to your first unknown slide and examine it under the microscope.

Then we carefully submerged the nitrocellulose sheet on the transfer buffer for about 5 minutes, and set up the transfer apparatus in the method shown in the lab outline week 2 step 3-6. Afterwards we took apart the transfer apparatus and detached the nitrocellulose sheet. Then we put the nitrocellulose sheet for 5 minutes into the blocking buffer, and we left nitrocellulose sheet in a container till week 3. The second part of week 2 was staining half our PAGE gel from week 1. Which consisted of pouring deionized water and use the shaker machine for 5 minute intervals, and repeating the the process three times. Then the Fresh Coomassie stain was added to the gel and was rinsed (UFV BIO 202 Lab,

The drops of crystal violets, approximately 15 drops, were flooded until the smear were all covered and then allowing resting for one to two minutes. After two minutes, the slide was titled over the sink and washed off, with the distilled water bottle, by aiming the stream of water above the smear. The specimen appeared blue-violet when observed with the naked eye. The drops of Gram’s iodine were applied on the slide until covered and then allowed to react for one minute or more. After the time elapsed, the slide was rinsed again with distilled water following immediate drops of Gram stain decolorizer added one drop at a time.

In this experiment, we wanted to investigate which surfaces in the school had the most bacteria, and we thought that the surfaces used more would have the most bacteria. For this experiment we used agar plates, cotton swabs and distilled water in order to see how much bacteria was on each surface. Cotton swabs were wet with distilled water and then the swabbed ws wied on a 2 inch space of the surface and twisted to cover the entire swab.once we swabbed the surface, we wiped the swab on the plate in a zigzag motion holding it upside down. The girls bathroom sink handle had 389 bacteria, and the boys bathroom by the football field had 0. We saw that places commonly used such as the bathroom sinks, door handles and railings contracted the most bacteria.

Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.

Just like eukaryote species, not all bacteria species are identical in the way they behave, grow or interact with their environment (Mujtaba & DeJarnette, 2012). Microbes can be unique in the way they grow on nutrient agar (NA) plates. How their colonies form on the agar plates can also be an exclusive property of specific bacteria species. Microbes can grow in the presence of different nutrients, environments and can also be inhibited by or thrive in the presence of specific agents in the medium. For example inoculating a blood agar plate with the organism, can indicate its hemolysis activity, which it will fall in one of the three groups alpha (α), beta (β), or gamma (γ).

You can grow microorganisms in liquid media or on solid media. Most bacteria can be grown in labs as long as the media contain a source of the major nutrients; carbon, nitrogen, sulphur, and phosphorous. They may also need to have other nutrients. These nutrients are made into a broth and the pH and salinity can be adjusted. The nutrient broth is placed in test tubes, which are plugged with cotton wool, capped with foil and then sterilised in an autoclave, at 121c for 20minutes. These tubes are then cooled before they are inoculated. To prepare the streak plates we dipped an inoculating loop into ethanol then placed it in the flame until the loop glowed red. Still holding the inoculating loop by its handle we removed the lid from

Microorganisms need energy and that is generated through these metabolic activities (Jurtshuk, 1996). Through this experiment, it is shows that all the bacteria experienced different metabolic activities in different mediums.

Choosing blood Agar plate and taking a sample of bacteria with a sterile loop then simple streaking it all on the blood agar plate the agar plate is incubated over night before reviewing the plate for any decolorization around the

We used ethanol when we used the crystal violet dye for instance. The ethanol allows the tightening of the peptidoglycan layer, making it parched and devoid of water, which in turn forces the stain to stay within the cell. When this happens, we can observe the cell accordingly under a microscope.

Final experiment is to test if the microbe had a red pigment at 25ºC. At the beginning of this experiment, we had inoculated the microbe to have more than one pure culture. Therefore, I inoculated a clean petri dish, allowed it to grow and saw the results.

This experiment is about bacterial growth. We will demonstrate a bacterial growth curve using a closed system. Bacterial growth usually takes up to 12-24 hours to get an accurate result so we will be monitoring this growth between two classes. We also used different methods to determine bacterial growth as well as a few different calculations. One way of receiving data is by using a spectrophotometer where we will record the absorption at a given time to create the bacterial growth curve. We also used the plate count method after performing a serial dilution to calculate the actual cell density at different times given. By using this method we can count the population number of the same given and see the maximum cell density

Streaking and spreading bacteria over the surface of the plate diluted the amount of bacteria diluted until the individual cells were streak and spread of the surface of the plate. From theses, individual cell a single colony arises. All cells in the colony genetically identical. However, the streaking and spreading was not quite properly performed, but there were some visible colonies that arise.

Every environment sample had its own growth rate and bacteria diversity. The primary reason may be that conditions are rarely optimum. Scientists who study bacteria try to create the optimum environment in the lab: culture medium with the necessary energy source, nutrients, pH, and temperature, in which bacteria grow predictably. Most of the strains used in the classroom either require oxygen for growth or grow better with oxygen. These bacteria will grow better on agar plates, where air readily diffuses into the bacterial colony, or in liquid cultures that are shaken. Since diffusion of oxygen into liquid depends on the surface area, it is important to have a large surface; volume ratio. This means that cultures will grow best in flasks in which the volume of liquid is small relative to the size of the vessel. Also another factor that affects growth is the nutritional medium. Bacteria grow best when optimal amounts of nutrients are provided.