Uniloacular Hydoatidosis Essay

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Uniloacular hydoatidosis is a zoonotic parasitic disease representing a major public health problem in many countries around the world, including Egypt. Close relation¬ships between dogs and humans appear to correlate with the high prevalence of the disease in endemic areas (Moro 2009) and camel is an influential reservoir of the disease. We selected the universal primers based on the highly conserved NADH dehyedrogenase 1 gene (Bowles and McManus, 1993) and our data indicated that the purified and partially sequenced PCR products generated 399 bp of NADH dehydreogenase 1 gene. The sequances were aligned by cluster grouping where the clusters aligned the most similar sequances firstly then progressively more distant groups of…show more content…
The exclusive finding of the G6 vareant in all camel and human isolates in Qalyobia Governorate, Egypt indicates the presence of a predominant transmission cycle in which the camel strain exist. Our findings confirms a previous study done using RAPD-PCR for characterization of human and animal hydatid cysts, it has been shown that human and camel isolates were the most related pair and camels are important hosts for the transmission of human hyadatidosis (Azab 2004). Similarly, performing the cycle sequencing and nucleotide sequence analysis identified the G6 genotype in 30 (96.8%) out of 31 human isolates in Cairo, Egypt (Abdel Aaty 2012). Although the camel strain G6 is traditionally considered as less infective to humans (Santivañez 2008; Casulli 2010), recent molecular findings (M’rad 2005; Osman 2009; Adel Aaty 2012) as well as ours suggest that the prevalence of infection of this genotype may be higher than previously thought. Among the ten genotypes of Eceohinococcus granoeulosus (EG) recognized worldwide, only 5 strains were known to infect humans including G1, G2, G5, G6, and G7 strains (Table 4). The most frequent strain associated with human CE appears to be the sheep strain (G1) and the highest rates of infection are recorded in communities involved in extensive sheep farming (Grosso 2012). Sequancing of our samples revealed
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