Unknown Bacteria 305 Summary

There are many types of bacteria that can be found in your bathroom. They might also be found on your toothbrush! I have been researching about what bacteria are lurking in my bathroom. I placed 6 petri dishes filled with agar in my bathroom. I left them there for 24 hours to collect bacteria samples.

In the last decade, the number of prescriptions for antibiotics has increases. Even though, antibiotics are helpful, an excess amount of antibiotics can be dangerous. Quite often antibiotics are wrongly prescribed to cure viruses when they are meant to target bacteria. Antibiotics are a type of medicine that is prone to kill microorganisms, or bacteria. By examining the PBS documentary Hunting the Nightmare Bacteria and the article “U.S. government taps GlaxoSmithKline for New Antibiotics” by Ben Hirschler as well as a few other articles can help depict the problem that is of doctors prescribing antibiotics wrongly or excessively, which can led to becoming harmful to the body.

The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others make antibiotics used today. This lab was completed by using the methods learned thus far in identification of bacteria.

The purpose of this study project was to carefully isolate and identify two unknown bacteria from a mixed culture. The ability to properly evaluate biochemical test results is also necessary for the identification to be successful. The goal was to apply all of the methods and techniques that have been learned in the microbiology laboratory course for the proper identification of unknown bacteria. A certain amount of bacteria that were used throughout the course were possible bacteria that could be found in a mixed culture. The bacteria that were identified in the mixed culture were Staphylococcus Aureus and Kocuria Rhizophila.

The biochemical tests, the Gram stain, the Bartholomew and Mittwer’s stain, the microscopic and macroscopic observations, and chemical sensitivity tests helped to identify the unknown bacteria as Citrobacter Freundii. Each biochemical test result maintained consistency with the

An unknown bacterium was handed out by the lab instructor. The methods that have been learned so far in identifying bacteria were applied to this unknown. Procedures were followed as stated in the lab manual and biochemical test handouts. The first procedure that was done was a gram stain followed by a streak of the unknown on a TSA plate in order to determine the gram reaction and observe the colony morphology. After that, specific biochemical tests were performed for gram positive, since unknown number five was determined to be gram positive rod. The other tests were performed in this order: Mannitol Salt (MSA) streak, Blood Agar streak, Catalase test, Nitrate Reduction test, and Phenyl

The objective of the investigation was to perform multiple experiments that would lead to the identification of an unknown bacteria. This bacteria was causing a urinary tract infection on a patient. A sample of the bacteria causing the infection was received and analyzed. The first step was to isolate the bacteria to insure that sample was free of contamination. A MAC plate, a PEA plate, along with a Nutrient agar plate where used to produce pure colonies by using the quadrant streak method on each plate. After a 24 hour incubation period the colonies where isolated. The bacteria collected was inoculated into slants, nutrient broth for cultivation. A sample from the MAC plate and the PEA plate were collected and gram

The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase, catalase, lactose and sucrose fermentation, Kugler/iron agar, nitrate reduction, gelatin hydrolysis, starch hydrolysis, manitol salt, MR-VP, citrate, bile esculin,

The purpose of this study was to identify the unknown bacterium using biochemical tests and various methods that had been learned from previous the microbiology laboratory class. Identifying the unknown bacterium was determined by separating and differentiating possible

There are many reasons for identifying an unknown bacterium. The purpose of this exercise was to identify an unknown bacterium from a liquid culture. We chose our unknown bacteria from a rack of test tubes with several different species of bacteria inside. I wanted to pick an unknown bacteria with a number easy to remember so I pick the test tube labeled “745”. Procedures were followed as stated in the lab manual written by Dr. Pedro J.A. Gutierrez.

The purpose of this report is to analyse the growth of the bacteria known as Citrobacter Freundii as well as distinguishing what antibiotics effect its growth. This will be done so by answering the following question after completing its associated experiments. This question includes: what antibiotics are most effective in denaturing the bacteria? It has been predicted that chloramphenicol will be the most effective due to the fact that its medical uses are treating meningitis which is an infection caused by Citrobacter freundii. After conducting the experiments it was found that the chloramphenicol antibiotic was the most effective in denaturing the bacteria, although streptomycin was also affective. However, none of the other antibiotics were able to halt the growth of the bacteria.

Different microbes can transmit and produce different types of diseases and infections. Having an unknown bacterium in the body can be a life and death situation. It is very important especially in the healthcare industry that providers are able to differentiate between organisms that are pathogenic and administer the appropriate treatment to their patients. Applying methods that were previously studied in lab, students must be able to isolate an unknown specimen by using laboratory techniques and biochemical tests.

Previously, detection and recognisation of bacteria were based on conservative tube-based biochemical reactions, and their results were compared to historical charts of expected biochemical reactions. Beacause of the need for quicker, simpler methods,

The purpose of this assignment was to correctly identify an unknown bacterium, labeled #76, which was provided by the lab instructor. After a series of experiments and lab techniques were performed, the unknown sample was classified as Citrobacter Freundii (C. freundii). C. freundii was first discovered in 1932 by Werkman and Gillen (Collier, Balows, & Sussman, 1998). It is a facultative anaerobic gram-negative bacilli identified in the Enterbacteriaceae family (Holt, Kreig, Sneath, Staley, & WIlliams, 1994).

Dr. Roberston’s research focused on how the bacteria can be transmitted and translocated by the displacement of plant residue. The bacterium overwinters on plant residue which can be moved by extreme weather and environmental conditions such as tornadoes, wind, and hail. Tornadoes can possibly translocate debris hundreds of miles which can possibly bring bacterium from Iowa to South Dakota and other neighboring states.