Essay about Unknown Rport on Bacillus cereus

2888 Words Nov 20th, 2013 12 Pages
Identifying the Unknown Bacteria: Bacillus cereus
Brittany Moy
Coastline Community College

Abstract
Bacillus cereus is a Gram-positive, facultative aerobic, spore-forming, rod-shaped bacterium that is widely distributed among the environment.
Introduction
The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also, knowledge of different bacteria helped others make antibiotics that are used today.
Bacillus cereus, a commonly occurring pathogen which can
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Results
There are many reasons for identifying an unknown bacterium. The purpose of this exercise was to identify an unknown bacterium from a liquid culture. We chose our unknown bacteria from a rack of test tubes with several different species of bacteria inside. I wanted to pick an unknown bacteria with a number easy to remember so I pick the test tube labeled “745”. Procedures were followed as stated in the lab manual written by Dr. Pedro J.A. Gutierrez.
Lab Day 1: After receiving my unknown bacteria, I streaked a TSA plate and incubated at 37°C for 48 hours. I then picked a single colony from the plate with my sterile loop and streaked a TSA slant and labeled it “Working Stock”. I did the same with another TSA slant and label the second one “Back-up Stock”. This would be the samples I used to complete the following procedures through the next four weeks to determine my unknown bacteria.
Lab Day 2: The first procedure that was done was a simple stain to identify the bacterial shape. Bacteria tends to be transparent, so a stain must be use to color the cells of the bacteria so it can be viewed under a compound microscope. After heat-fixing three separate loopfuls of my unknown bacteria onto a slide, I used Methylene blue, Safranin, and Crystal violet to stain the three different samples. After rinsing the slide and observing my finding under the microscope, I

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