Urinary Infection Paper

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Doris a 64-year old women with a kidney infection and history of urinary tract infections gave a urine sample. Unknown culture #304 was isolated from the sample provided. From the broth culture a reserve stock and working stock was prepared. The reserve stock was made by the inoculation of a nutrient agar plate. The nutrient plate was divided into four sections. The working stock was made with the quadrant streaking method by the inoculation of a nutrient agar plate. In order to observe the morphological characteristics of unknown culture #304 tests were performed. The tests go by the protocols of Brown. Inoculating and incubating two agar slants in two different temperatures 27 C and 37 C was conducted as the first test to determine the optimum temperature for incubation on unknown#304. Motility test was the following experiment using a motility tube to see if the organism was motile or non-motile. A gram stain was done to determine the shape of the organism. An EMB plate was inoculated with the unknown culture as well as a PEA plate. This was used to help determine if the organism was gram-negative or gram-positive.…show more content…
In one of the tubes the nutrient broth was used to see the amount of growth on the sediment, subsurface and surface of the tube. A nutrient agar was used to examine the growth’s form, color and opacity. Another method colony morphology was done to show the color, size, form, margin and elevation of the unknown culture #304. To determine the oxygen requirements for the organism a FTM was used. A gelatin stab was performed to observe if liquefaction would occur. This test will show if the unknown culture is gelatinous. The last test to see the cultural characteristics was done to see how tolerant the organism is to salt. Four agar plates were inoculated to see the different osmotic
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