Usage Of Restriction Enzymes Of Dna Fingerprinting

1237 Words5 Pages
Usage of Restriction Enzymes in DNA Fingerprinting Analysis Jaclyn Napoli October 30, 2014 Cellular Processes Lab BSC 2010L.905 Lab Partner: Jessi Grillo Material and Methods To start off the experiment, 4 microtest tubes were labeled reaction tube 1 through 4. Using a micropipette, 10 microliters, ul, of Enzyme Reaction Buffer was dispensed into each of the 4 labeled reaction tubes. In reaction tubes 1 and 2, 15 ul of suspect 1’s DNA was added. Reaction tube 1 had 15 ul of Enzyme 1 added to it, and reaction tube 2 had 15 ul of Enzyme 2 added to it. 15 ul of suspect 2’s DNA as added to reaction tubes 3 and 4. In reaction tube 3, 15 ul of Enzyme 1 was added. Then, in reaction tube 4, 15 ul of Enzyme 2 was added. Finally, 2 more microtest tubes were labeled as crime scene samples. The samples contained DNA from the crime scene and each the tubes had either Enzyme 1 or Enzyme 2 added to it. The microtest tubes were then capped and tapped lightly to allow the ingredients to mix. The reaction tubes were submerged in a 37°C, ice water, for 45 minutes. Once complete, 5 ul of 10x gel loading solution was added to all 6 of the microtest tubes. Refer to Table 1 for a quick overview. Table 1 Summary for Restriction Enzyme Digestion Process. While the tubes were being cooled, a casting gel was made. The cast was made by combining 0.5 grams, g, of agarose and 50 milliliters, ml, of TBE buffer into a 250 ml flask. The TA added ethidium bromide to the solution to allow
Open Document