Use Of Antibiotic Resistance For A Large Number Of Infections

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Maddie Ward Period 6 January 22, 2015 Transformation Lab Abstract: Ampicillin is a beta-lactam antibiotic which can be used to treat a large number of infections. For example, Escherichia coli (E. coli) bacteria is terminated by this specific antibiotic. Ampicillin interferes with the formation of bacterial cell walls and thus kills newly dividing cells that must form new cell walls. Plasmids contain genes that create antibiotic resistance to their host cell. The pGlo plasmid contains an Ampicillin resistance gene. Therefore, bacteria that take up the plasmid and transform become resistant to Ampicillin. To carry out this experiment, my colleagues and I took four petri dishes containing the bacterial host cells (E. coli) on each and the Ampicillin on two of those four. Through a tedious process, we added the pGlo plasmid to one petri dish of just the host cell and one petri dish of the host cell and the Ampicillin antibiotic. When examining the results of our experiment, we noticed that both petri dishes containing zero Ampicillin entirely submerged in a lawn of the Escherichia coli bacteria, the petri dish containing no plasmid but some Ampicillin displayed zero growth, and the petri dish containing the plasmid and Ampicillin showed individual colonies of the E. coli bacteria glowing under a UV light. The reason this occurred is because the petri dishes that showed a lawn of bacteria contained no Ampicillin so there was no antibiotic to kill the bacteria off. The petri
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