Olivia Silvestri Due: 10/19/15
Using a Compound Microscope Lab Report E Period
Abstract
The purpose of this lab is to learn about the parts and uses of a compound microscope. It is also to learn how to properly use and take care of the microscope. This lab showed me what materials and parts of the microscope I should use when examining an object.
Introduction
The microscope was invented by a father and son, Hans and Zaccharias Janssen. They were once looking at the lens of a magnifying glass and decided to put the lenses in a tube. When they looked through the tube, they noticed that the object underneath the magnifying lenses, was enlarged. That was the first invention of the compound microscope. Overtime, more advancements and improvements were made by other scientists to this microscope (1. microscope-microscope.org). A scientist that contributed to the cell theory is Robert Hooke. Robert Hooke was the first person to use the word "cell" to describe the basic unit of life. He was also known for his observations of thinly sliced cork. Robert Brown contributed to the cell theory by discovering the nucleus of a plant cell (2. brighthub.com). The scientist Schleiden, made the statement about cells that all plant tissues were made up of cells and that cells are the basic building blocks of all plants. Schwann discovered that both plants and animals are made up of cells.
The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in
Hypothesis: The purpose of this experiment was to investigate the question will different food sources affect the level of activity of detoxification enzymes in bean beetles? The class alternate hypothesis is different food sources will affect the level of activity of the detoxification enzymes in bean beetles. The null hypothesis is the different food sources will not have any effect on the level of activity of the detoxification enzymes in bean beetles. Experimental design: The independent variables in this experiment were the types of beans (bean 1 was mung beans and bean 2 was adzuki beans) and enzymes assays used.
In this project, C. Elegans are hermaphrodite worms that will be used since they are easy to maintain in lab, as well as have short life cycles. The gene that the project attempted to knockdown in C. Elegans with RNAi treatment is the unc-22 gene. RNAi disrupts gene expression in the presence of double stranded RNA (dsRNA) that is complementary to target gene sequence. The unc-22 gene codes for a muscle protein called twitchin in wild-type worms. The Unc-22 is required for muscle regulation and maintenance in C.Elegans. To verify that the RNAi treatment worked, would check the unc-22 mRNA levels in the worms, in addition to phenotype observation.
It was then during the 1950’s that Zacharias Jansen and his father, Han Jansen, created the first high-powered compound light microscope. Images could be magnified up to 9x which was a novel feat at that time. Zacharias and his father created the device after experimenting with glasses and discovered that by placing multiple lens in a tube they could increase magnification. Unfortunately, the microscope lacked the high resolution we have come to expect in modern-day light microscopes. The images produced by the Hans’ primitive microscope was extremely blurry and had limited
The purpose of this lab was to practice proper handling of the light microscope, learn the names and functions of the light microscope parts, acquire skill in using the light microscope by carefully following all directions, prepare a wet mount, and locate objects under low and high power magnification. In addition, we will learn to position objects when viewed with a microscope, adjust the diaphragm correctly to achieve proper light under low and high power, learn to locate objects at various places in the “depth of field” and use stains (iodine) as an aid.
2. When 2.00 g of NaOH were dissolved in 49.0 g water in a calorimeter at 24.0 ˚C, the temperature of the
In the 1800’s there was a scientist who worked on cells and how they came to be, and why are there so many of them?He believed that only cells came from other cells.He was the first person to discover cells and yet he wanted to know all about them.He was the first person to know about all living things being composed of
The mean voltage of the battery terminals while connected to the identification resistors is presented in Figure 4 12. These samples have been pulled out from the voltage sensor of the PEB panel. The voltage decreased as expected from 12.53 to 12.5 during first 20 seconds of connection to the
By using their microscopes, they found that every living plant and animal they examined was made of cells. As microscopes were improved, scientists were able to see smaller and smaller organisms. They found that no matter how large or small the organism was, it was made of cells, leading to cell theory. For example, a German biologist, Theodor Schwann discovered that all plant and animal cells were divided into cells by looking through his microscope. He also discovered that the cell is the basic unit of organization in organisms. Cells can be grouped together to form tissues, which can in turn be grouped together to make an organ. Organs can be grouped together to form a system, which is part of an organism. He was able to use microscopes to see the ways that cells work and help to determine which kind of microorganisms (bacteria) is causing the disease and making people ill. This is particularly valuable in the study of the components of organisms, where physicians are able to overcome a treatment of method to kill disease cells and restore people¡¦s health. The microscope revealed not only the cellular structure of human tissues, but also the organisms that cause diseases. The discovery of cells led scientists to study cells and discover more information about cells; this, allowed scientists to find ways to prevent or cure diseases. The use of microscopes has made many
October 17, 18, and 19, samples were collected from multiple sites along the BSR. The class was split into groups, and samples were collected from seven separate locations along the river and WWTP. There was also a sample collected by the S which is located between sites four and five. For each of these sites, there were ten groups from other labs that also collected a sample from the BSR. At site two of the river, the location included multiple sources of possible contamination. A drainage site was located 200 yards upstream, along with a small PVC drainage pipe next to the collection site. Not only was there drainage running into the river, the site was under a bridge, and contained other trash scattered throughout the area. The
It is consisted of eyepiece, eyepiece tube, aperture, objective lens, Iris diaphragm, illuminator and fine focus knob. The sample are put on stage to view it carefully by adjusting the aperture and focus of the microscope. Part A In this
But first, let us talk about the discovery of cells and the cell theory. Robert Hooke, an english scientist, was the man who first discovered the cell in 1665, proof being a book he released at that time called Micrographia. In this book, Hooke gave 60 observations of random objects under a compound microscope with a magnification of 30x. Because of this, he was not able to see the internal structures in the cell, like nuclei and vacuoles, and what he proclaimed to be cells were just empty cell walls of plant tissues. He shared his observations with The English Royal Society, until they started receiving letters from a scientist named Anton van Leeuwenhoek. The letters stated that Anton made use of a microscope containing improved lenses that magnified objects up to 275x, enough to identify the living parts of a cell. He kept on sending
Cells, they are the building block of all life, they are what make up the entirety of living things on Earth and without them living things would cease to exist. It is because of this that cells are considered to be the most important known unit of life and are studied so often. Cells were first observed in the mid 17th century by English physicist and microscopist Robert Hooke using on of the earliest versions of the microscope. (Biologyreference.com, 2017) Hooke’s observations led to further investigations and studies into the topic of cells resulting in many major breakthroughs in the field of biology. Possibly the biggest of these breakthroughs is credited to have being made by Theodor schwann, Matthias Schleiden and Rudolph Virchow,
NOTE: Answer Question A only if you used a compound light microscope for this experiment.
Some are so large that they are housed in silos several stories high while others are small enough to fit in the palm of a hand. These instruments have given scientists a new look at the world around us.