Using Garlic Root Tips to Observe Mitosis

1030 WordsOct 27, 20115 Pages
Mitosis is a cellular process that replicates chromosomes and produces two identical nuclei in preparation for cell division, from the original cell two cells are derived, each of which possesses the same genetic material. Mitosis has five phases: interphase, prophase, metaphase, anaphase and telophase. Interphase The DNA duplicates during interphase to prepare for mitosis. Chromosomes are not clearly discerned in the nucleus. Prophase Chromatin in the nucleus begins to condense and becomes visible in the light microscope as chromosomes. The nuclear membrane dissolves. Microtubules attach at the kinetochores and the chromosomes begin moving. Metaphase Spindle fibers align the chromosomes along the middle of the cell nucleus. This line is…show more content…
The first 2 phases of mitosis, interphase and prophase, were observed in the cells of the root tips. The experiment did not reveal any cells undergoing metaphase, anaphase or telophase stages of division. If time had allowed for it the experiment would have been more accurate if the search was carried out for all the material on the slide. 1. During the 20 minute stain time (Step 6) it is important to have the students discuss what the cells are doing. Since these cells are in the root tip, they are rapidly dividing. During normal cell activity the chromosomes are unwound and too thin to be seen. During cell division, chromosomes thicken, take up stain and can be easily observed. The students should also try to come up with reasons why cells divide (possible answers: to grow, to repair or replace damaged cells, to reproduce, or to differentiate in the cell cycle of multicellular organisms). Although it is better to have the students elicit these, it may be necessary to give them some of the reasons since this is an introduction. Also at this time explain what is happening in interphase. Cells need to replicate the chromosomes before

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