Using Gel Electrophoresis And Dna Fingerprinting To Analyze

2108 WordsApr 14, 20179 Pages
Using Gel Electrophoresis and DNA Fingerprinting to analyze DNA samples Laquandria M. Gibson April 14, 2017 BSC2010L Section #22 Sarah Ellmallah Introduction All cells contain a complex structure known as deoxyribonucleic acid (DNA). DNA is a chemical that determines how we are. The multiple combinations of its components are what makes a difference in each person. Long molecules of DNA are organized into chromosomes, which are grouped into 23 pairs. Then the chromosomes are broken down into short segments of DNA known as genes. A gene is a basic physical and functional hereditary unit. Every gene contains a sequence of DNA that occupies a locus on a chromosome (Upadhyaya, 2017). Genes act as instructions to make proteins, varying…show more content…
And gel electrophoresis includes the movement of charged molecules in a buffer solution (Cassill, 2015). The gel is composed of a buffer solution containing agarose, a polymer that easily forms a gel-like material at room temperature (Cassill, 2015). Even though DNA fingerprinting does not compare all of DNA’s structure, it does compare the different cuts made by restriction enzymes, molecules that attach to DNA at the recognition sites and results in cutting the DNA strands (Upadhyaya, 2017). Common restriction enzymes used in DNA fingerprinting are HIND III and ECOR I and because, as mentioned, all alleles are different in their base sequences, recognition sites for restriction enzyme tend to vary based on the individual (Upadhyaya, 2017). Materials Restriction Enzyme Digestion In order for DNA samples from suspect one and suspect two to be digested by two different restriction enzymes, four reaction tubes were required, labeled 1-4. In each reaction tube, with a micropipette, ten µL of reaction buffer was used. All the samples were prepared based on the given chart (shown below as Table 1) (Upadhyaya, 2017, p. 58). As far as all four having the same enzymes that was the end, so to not cross-contaminate, the micropipettes tips had to be changed each time (Upadhyaya, 2017). The reaction tubes one and three contained 15µL of enzyme 1 and enzyme 2 was added to reaction tubes two and four. Then, reaction tubes one and two were filled with 15µL of
Open Document