Using Mesoporous Silica Nanoparticles From Chemotherapeutic Cells With Chemotherapeutic Drugs And Sirna Against Abcg2 Mediated By Thermo / Ph

3419 WordsJun 27, 201514 Pages
Targeting CD133+ laryngeal carcinoma cells with chemotherapeutic drugs and siRNA against ABCG2 mediated by thermo/ph sensitive mesoporous silica nanoparticles Authors Xinmeng Qi,a Dan Yu, *a Bo Jia,b Chunshun Jin,*a Xueshibojie Liu,a Xue Zhao,a Guangxin Zhangc Affiliations and addresses a Department of Otolaryngology Head and Neck Surgery, the Second Hospital, Jilin University, Changchun 130041, China b Department of Medical Oncology, Cancer Institute/Hospital, Chinese Academy of Medical Sciences(CAMS)& Peking Union Medical College(PUMC), Beijing 100021, China c Dapartment of Thoracic Surgery, the Second Hospital, Jilin University, Changchun 130041, China Corresponding author: Name: Chunshun Jin E-mail: chunshunjindan@163.com Tel: +86…show more content…
Since positively charged nanoparticles could easily uptake nucleic acids, we examined the possibility of using this new DDS to simultaneously deliver different chemotherapeutic drugs and siRNA targeting ABCG2. Our results demonstrated that both anti-tumor drugs and siRNA against ABCG2 were successfully delivered into CD133+ cancer cells of laryngeal carcinoma by loaded MSNs. Down regulation of ABCG2 significantly enhanced the efficacy of apoptosis induction by chemotherapeutic drugs in laryngeal carcinoma cells. Furthermore, the chemotherapeutic drug and siRNA loaded nanoparticles also inhibited tumor growth in vivo in a laryngeal cancer mouse model. Key words: CD133+; laryngeal carcinoma; ABCG2; siRNA nanoparticle Introduction In 2012, an estimated 156,877 new cases of laryngeal cancer were diagnosed worldwide. In the same year, this cancer was responsible for 83,376 deaths worldwide[1]. Surgical resection followed by chemotherapy remains to be the standard treatment for laryngeal carcinoma[2]. However, postoperative malfunction or completely loss of phonation may cause great pathological and psychological disorder to patients who may then loss their social adaptability. Moreover, a considerable number of patients are threatened by the risk of local recurrence and metastasis. Chemotherapy is advantageous in reserving laryngeal function and structure[3]. However, multidrug
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