Using Radiotherapy On Hepatoblastoma ( Hb ) Is The Most Common Malignant Embryonal Liver Tumor
817 Words4 Pages
Background/Aim: Hepatoblastoma (HB) is the most common malignant embryonal liver tumor in the pediatric population and the incidence is increasing. In order to evaluate the feasibility of utilizing radiotherapy on Hepatoblastoma (HB), here we conducted a vitro experiment to detect the radiosensitivity of a HB cell line Huh-6. Materials and methods: Cell proliferation, morphology change, cell apoptosis and cell cycle of Huh-6 cells received gradient X-ray irradiation (0 Gy, 1 Gy, 2 Gy, 4 Gy and 8 Gy) were systemically studied with CCK-8 assay, microscopy, Annexin V/7-ADD and PI single staining respectively. Results: The inhibition of proliferation and survival fraction, the percentage of deformed cells, and the increase of apoptosis and cells arrested in G2/M phase were positively correlated with the irradiation dose. Conclusion: This Huh-6 cell line is radiosensitive in vitro and the efficiency of inhibition is positively correlated with the irradiation dose received, indicated the potential of radiotherapy on the treatment of HB. .
Hepatoblastoma (HB) is a rare tumor comprising only around 1% of all pediatric malignancies (1, 2), with an incidence of 9.8 and 6.1 per million in age groups under 1 year and 1 – 4 years respectively (3–5). However, HB is the most common malignant embryonal liver tumor in the pediatric population and the incidence is increasing at a rate of 1.2-1.5 cases per million each year, due to the potent risk factor of rising