Using The Henderson-Hasselbalch Solutions Of Specific Ph

In this lab, the purpose was to determine the stability of a substance after adding an acid or a base. The results claim that liver and buffer are the most resistance to change in pH. Looking at figure 3, buffer and liver both maintain a stable pH even with the addition of an acid or base. However, potato and water have less buffer in them since their pHs did change. In figure 3, the potato acid’s pH level decreased by two, and the potato base’s pH level increased by two. The level of pH of a water acid decreased by 4, while the water base’s pH increased by 5. These results all tie to the fact that buffer is a substance that maintains a stable pH; the presence of buffer in organisms help maintain homeostasis by binding or releasing hydrogen

1. To titrate a hydrochloric acid solution of “unknown” concentration with standardized 0.5M sodium hydroxide.

The procedure of this lab is to determine if liver and potato cells contain natural buffers that resist large change in pH as 1. NaCl or 1. NaOH are added to the solution.

A buffer is a solution that resists changes in pH when H+, OH-, or H20 is added. By using standard lab equipment, a lab pro diagnostic tool, and acidic and basic solutions, the pH can be found. By recording the pH while adding a base or an acid gradually to a buffer solution you can find the capacity of each buffer to resist drastic changes in pH. The best buffers will keep a solution from becoming either too acidic or basic with the addition of a strong base or acid.

• Dilute some of the enzyme stock with buffer A to make a 4 mg/ml solution.

weak bases). After ranking the pH of these solutions, you will then test your predictions in the laboratory.

To start out this study the difference between acids and bases has to be identified. Acids have very low pHs and have a high concentration of hydronium ions, while bases have a high pH and have a high concentration of hydroxide ions. The difference between strong bases and acids, and weak bases and acids is the amount of dissociation. Strong bases and acids dissociate a large amount and let go of their ions in solution, while weak bases and acids may only let go of some of their ions. This is important because if the unknown solutions aren’t strong acids or bases then using their ions to calculate the pH of the solutions will give false results (Diffen 2012).

1. We measured 2 mL of diluted hydrogen peroxide (the substrate), 1 mL of guaiacol (the product indicator), and 1 mL of neutral buffer (pH 7) with a syringe and disposed it into tubes 1, 2 , 4, 9, 11, and 12.

To improve the results from the experiment buffer solutions that were not whole pHs could have been used e.g. pH 4.5, 5.5 etc. This would have provided more reliable results as a wider range of results would have been produced. Using pHs with decimals would also help to more accurately determine the optimum pH as the optimum may have been above or below the pH stated in the hypothesis; 8. In this experiment however the optimum is taken at 8 because the graph does not rise again.

Table 2: Consists of color extract taken from a red cabbage for a natural indicator. The pH reading that was measured by using the pH meter and the result of the pH reading to determine whether the solution was acidic or basic.

To make the buffer solution you need 0.2 mol dm-3 of Na2HPO4 and 0.1mol dm-3 of citric acid this will give 100cm3 of buffer. Here is how to get the different pH in the buffer solutions:

We will observe the reaction of a buffer solution with added acids or bases. We will then evaluate buffering capacity in response to additions added from dilute acid and bases to the buffer.

In this experiment, a saturated calcium sulfate was already made and ready to use. 25.00 mL of this solution was then mixed with 10 mL of an ammonia buffer and 1 drop of

The pH of a solution is the measure of the concentration of charged Hydrogen ions in that given solution. A solution with a pH lower than seven is considered to be acidic. A solution with a higher pH is a base. It is very important for organisms to maintain a stable pH. Biological molecules such as proteins function only at a certain pH level and any changes in pH can result in them not functioning properly. To maintain these constant pH levels, buffer solutions are used. A buffer solution can resist change to small additions of acids or base’s. A good buffer will have components that act like a base, and components that act like an acid.

For this experiment, titrations on a weak acid, acetic acid, and a buffer were performed. Acetic acid was titrated with NaOH in order to observe the half-equivalence point as well as the equivalence point. Then, the buffer and the buffered acetic acid solution prepared faced additional titration with NaOH and HCl to evaluate the differing buffering effects following the addition of a strong acid and strong base. Finally, the buffer’s buffering capacity was calculated. If the experiment were to be repeated, it would be interesting to observe the buffering effects following a titration between a weak base and a buffer instead with greater concentrations. The change in the concentration following the preparation of buffer with a weak base and its conjugate acid would pose for an interesting experiment to observe an increase in the buffering capacity.