Introduction
Uv radiation is damaging to the cells that are in our body. There are three types of UV radiation lengths; UV-A, UV-B and UV-C. UV-A and B can have the most damaging effects when it is exposed to soft tissue such as a person’s eyes and/or skin causing premature aging and possibly cancer (2). The use of sunscreen among other items are used to limit the exposure to the radiation. In this experiment wild type yeast was exposed to UV radiation in different time sequences. Yeast was incorporated into this experiment because their genome is similar to the human genome. Yeast can also give insight to basic human diseases because they are easily manipulated. In a previous experiment like this one stated below, wild type yeast was exposed to UV radiation and the results shown that the longer the exposure to radiation the less percent survival there is.
The experiment was recreated but the use of saran wrap was placed over the petri dishes. Saran wrap was decided because of its refractive properties. The notion was, the more layers or saran wrap added, then the survivability of yeast will improve.
Methods
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Inoculation of each plate was done one by one by adding 100ul of the diluted yeast onto each plate and spreading it around. For the experimental group cling wrap was used in a series of layers ranging from one layer, two layer and three layer. The yeast plates were then transferred to the transilluminator and exposed to the UV radiation in intervals of 20, 30 and 40 seconds for the first experiment and 10, 15, and 20 seconds for the second experiment. The control group was inoculated in the same manner as the experimental. They too were wrapped in cling wrap, however they were not exposed to the UV
Dermatologists, physicists, and scientists conducted a workshop of UVA treatment, and the workshop’s findings are presented in the New England Journal of Medicine. A previous president of the American Academy of Dermatology wrote an article titled “UVA1 is Often A1” to discuss their findings. The workshop found that medium or high-dose UVA1 works well with morphea, urticarial pigmentosa, atopic dermatitis, dyshydrotic dermatitis, subacute pruigo, and systemic lupus erythematosus. These diseases are treated by UVA rays because the wavelength is longer, which means there is less energy delivered to the skin and the greater penetration of photons (Dahl, 2012). Dermatologists even use sunbeds with higher intensity than regular tanning beds in their offices. For example, Windsor Dermatology uses four different types of light therapy to treat the skin condition psoriasis. One type of light therapy is that of UVA. The anti-inflammatory properties of ultraviolet light aid in slowing the growth of psoriasis effected skin
UV light at 260nm causes the most damage to DNA because that is the amount of light that DNA absorbs the most light. No, UV light does not affect both prokaryotic and eukaryotic cells the same way. For example, in eukaryotic cells there are three different ways DNA can be repaired (photoreactivation, post-replication repair & excision repair), whereas in prokaryotes excision repair mechanism is used.
We hypothesized that the amount of UV radiation exposed has a negative correlation with yeast genetic stability, which ultimately determines the number of yeast colonies. In this experiment, yeast cells were treated with UV radiation, protected by photoprotective agents with different strength (in ascending order: Sunscreen #2, #1, aluminium foil). After letting it grow for seven days at 37 degrees, some grown colonies were counted to determine the effectiveness of photoprotective agents. After comparing collected results with the controlled group, we conclude that sunscreen #2 absorbs nearly zero UV radiation (0 yeast growth). Sunscreen #1 has some photoprotective effect (observed yeast growth). Theoretically, aluminium foil should be the
The purpose of the testing and experiments conducted was to understand how the glycolysis in cell respiration works, to understand how the process of fermentation in yeast works and see the difference between fermentation and respiration in the yeast. Yeast is something that may not be alive, but when it is put in water with sugar, the yeast will take up the sugar and use the energy stored in the sugar molecules and carries out the processes of life. Living organisms are all similar in that they can take energy from their environments.
Have you ever felt the “false sense of security that [you] may not feel under direct sunlight” from a tanning bed (Jeffrey)? Your body relaxes, you feel serene, and you get a golden glow. Some teens strive for this feeling...and the tan. Tanning is the skin's response to injury, according to Arielle Kauvar, M. D. The exposure of UV rays over time can cause skin cancer. Skin cancer is the growth of abnormal growth of skin cells that forms in the tissue of the skin (Skin Cancer). Melanoma, the most dangerous type of skin cancer, can come from UV rays. From 1998- 2008, the number of cases of Melanoma has increased by about
Individuals who get a considerable measure of presentation to bright (UV) beams are at more serious hazard for skin cancer. Sunlight is the fundamental wellspring of UV beams, yet you don't need to keep away from the sun totally. What's more, it is hasty to remain inside if it would shield you from being dynamic, in light of the fact that physical action is imperative for good wellbeing. Yet, getting excessively sun can be unsafe. There are a few stages you can take to confine your presentation to UV
They’re such a good model organism for many reasons: including that they are eukaryotes (as are we), and that we have completely sequenced their DNA through many years of research. According to Botstein, we have known the complete DNA sequence of Saccharomyces cerevisiae (the yeast we will work with in this lab) since before 1996. Yeast is also great to work with because of their availability and easily observable growth. (Botstein, 1997). Due to the many similarities between human and yeast cells, the effects of damage such as radiation on yeast cells can be correlated to the effects that it will have on other types of cells, such as human cells.
The lab group conducted three separate experiments to test the effect of ultraviolet radiation on the survival of yeast cells. These experiments are significant because ultraviolet radiation can damage the DNA of human skin cells and eventually lead to skin cancer. The yeast cells were prepared and then exposed to UV for various increments of time. The first experiment was a comparison of a wild type strain versus an unknown type. The hypothesis was that if the unknown yeast has better adapted to ultraviolet radiation, more unknown cells would survive. The wild-type strain survived substantially more than the unknown. The second experiment introduced “protections.” The group covered the yeast samples with a thick black film or a sample of distilled water. More cells under the black film outlasted the cells under the distilled water. The third experiment covered the samples with a thinner, red film or a general sunscreen with SPF 70. More cells covered by the sunscreen lived than those under the red film.
This experiment yielded different results than initially expected. The control tests indicated that a lack of UV exposure results in freely growing S. marcescens, while UV exposure quickly kills most of the bacteria. Taking these results in mind, it was expected that when exposed to the bacteria guarded by radiation protecting sunscreen, most bacteria would survive in the plates containing the highest strength of sunscreen. However, the least growth was seen in all three trials of the SPF 70 plates. Meanwhile, the plates with SP 15 and 30 showed the most growth, while the plate with the SPF 50 showed a large amount of growth slightly
The sun is a vital part of human existence and we wouldn’t be where we are today without it, it is unavoidable. The sun is what keeps so many living things going and provides us with a natural source of Vitamin D which helps keep your bones healthy. However, the sun can also be harmful, the sun emits Ultraviolent, or UV Rays. There are three different types of UV Rays, UVA, the most common form of sun exposure, UVB, less sun exposure but more intense, and UVC, which is blocked by the Earth’s ozone layer. Although we cannot see
Some people think having a tan gives them a "healthy glow." But a tan really shows that the skin is trying to protect itself from sun damage. Sun damage can lead to premature aging (wrinkles!), eye damage, and skin cancer.When a type of radiation (ultraviolet [UV] radiation) is exposed to the skin, the skin responds by producing melanin. Melanin is a dark substance that helps prevent the body from taking in too many harmful sun-rays that can damage skin.The sun produces two kinds of rays, UVA and UVB. UVB rays are around all year and UVA rays are mostly present only during the summer. UVA rays are regarded as "safer" and they cause the skin to age, while UVB rays cause the skin to burn. However, both of these types of rays are harmful and
Spring is finally here, and Spring Break and the Senior Prom are around corner. The teenagers and the college kids are planning a drive to the tanning salons or a day at the beach. The media has instilled in women that tan skin is a sign of youth and sex appeal. Some people go to the tanning salons and others spend hours outside in the sun to achieve the sun goddess look. Many don’t realize the dangers that come with the sun or a box with light bulbs that has more UVA radiation than the sun does. From fair to dark skin, people are being destroyed by the same UVA radiation that gives people that “sexy glow”. Being in the sun is inevitable, but people can still protect their skin from many forms of skin cancer by doing the simplest things.
Section 2: The article is referring about skin cancers, most specifically, Basal Cell Carcinomas. The article states the facts in a much more detailed manner, stating that indeed people who expose themselves to ultraviolet rays for prolonged period of time, some form of abnormalities such as skin cancer may occur.
Every hour at least one American will die of skin cancer caused by exposure to the sun. These statistics are alarming and most people are unaware that the sunshine that we all love so much is in fact a threat to our health and good looks. It is up to us to educate ourselves and our children on the harmful effects of sun exposure on the human body like premature aging, physical damage to the body, and cancerous diseases.
Conclusion: The yeast was tested multiple times at different concentrations of 100%, 50%, and 10%. As