BIOLOGY LABORATORY REPORT Name : Amirah Binti Shaharuddin IC No. : 930428-08-5326 Student ID : 2012400466 Group : 13M1 Title : The Vitamin C Content of Fruit Juices Lecturer : Madam Rita Rohaizah binti Sohari TITLE: THE VITAMIN C CONTENT OF FRUIT JUICES INTRODUCTION Vitamin C which is also known as ascorbic acid is an essential nutrient for human. It is a water-soluble vitamin that is necessary for normal growth and development. Water-soluble vitamins dissolve in water. Leftover amounts of the vitamin leave the body through the urine. That means a continuous supply of vitamin is needed in daily diet. Vitamin c is an odourless white solid with the chemical formula of C6H8O8. Vitamin C is one of many …show more content…
17. The volume of carton fruit juices used to decolourise the DCPIP solution is calculated and recorded in Table 3. RESULT Initial concentration of vitamin C solution =Mass of vitamin C gVolume of solution cm3×100% =1 g100 cm3×100% =1 % The final concentration of each vitamin C solution can be found by using this formula : Final concentration of vitamin C solution (%) =Initial concentration of solution %×Initial volume of solution (cm3) Final volume of solution (cm3) Initial concentration of solution (%) | Initial volume of solution (cm³) | Volume of distilled water added (cm³) | Final volume of solution (cm³) | Final concentration of solution (%) | 1.0 | 20.0 | 80.0 | 100.0 | 0.2 | 1.0 | 20.0 | 30.0 | 50.0 | 0.4 | 1.0 | 20.0 | 20.0 | 40.0 | 0.5 | 1.0 | 20.0 | 5.0 | 25.0 | 0.8 | 1.0 | 20.0 | 0.0 | 20.0 | 1.0 | Table 1 : Concentration of vitamin C solutions Concentration of vitamin C (gcm-3) | Volume of vitamin C solution to decolourise 1cm3 of DCPIP solution (cm3) | 0.2 | 40 | 0.4 | 35 | 0.5 | 30 | 0.8 | 25 | 1.0 | 20 | Table 2: The volume of vitamin C solutions that decolourises 1cm3 of 1% DCPIP solution. Type of fruit juice | Volume of fruit juice needed to decolourise 1 cm³ of DCPIP solution (cm³) | Fresh lime | 11.0 | Fresh guava | 79.0 | Fresh orange | 20.0 | Fresh pineapple | 44.0 | Carton lime | 73.0 |
Vitamin B and vitamin C are vitamins that are soluble in water, also known as water soluble. Whilst, vitamin D and vitamin k belong to a group that are soluble in fat which are known as fat soluble.
Vitamin C is an essential in the body's defenses against infection. Susceptible children will not tolerate relatively unimportant infections and this can lead to prolonged illness and in the case of behaviorally disturbed children of symptoms previously well managed. Vitamin C is an extremely safe substance which is immensely beneficial to the brain and body in a multitude of ways. Its potential for preventing and treating autism has barely been touched. Vitamin C is heavily concentrated in the brain, but its exact role in brain function has not been fully understood. A recent search turned up 400 references referring to vitamin C and the effects of the brain, but the mystery still remains.
* Baby need Vitamin C is necessary for a normal growth and development immune function and help with iron.
3.6.3. 2, 4 – D (2, 4–Dichloro phenoxy acetic acid) stock solution (1mg/ml): 10.0mg of 2.4-D being weighed and dissolved completely in 1N NaOH to a final total volume
Vitamin C is needed for a baby to develop well as it helps our bodies fight off infection and disease as well as helping to heal wounds. It can also prevent anemia. Some lactating mothers may need more vitamin C than others, especially if overcoming a cold, surgery or some form of injury. The dose needed is 115 mg or 120 mg per day. Food sources in which vitamin C can be found are citrus fruits, broccoli, Kiwi, cauliflower and bell peppers.
The Red #40 was mixed with 10 mL of DI water, the solvent. The volume of stock solution needed to prepare these solutions are shown in the second column of Table 3. After obtaining the appropriate micropipettor, 200 uL, it was used to measure the aliquot of the stock solution. This solution was transferred into a volumetric flask and diluted with the 10 mL of DI water. DI water was used as the blank and the solutions were then placed inside the cuvettes which were then placed into the Vernier pH spec-trophotometer. The absorbance and λmax of each solution were then recorded as seen in the third column of Table 2. These values were then plotted on a linear regression as seen in Figure 1. Next, 0.50 mL of maraschino cherry juice was obtained by using the appropriate micropipet-tor, 100 uL, of the aliquot of the stock solution. This was transferred into a volumetric flask and diluted with nanopure water until the solution reached 25 mL. Nanopure water was used as the blank and the solution was then placed in a cuvette which was then measured inside the Vernier pH spectrophotometer. The absorbance and λmax of this solution was then recorded in third column of Table 3. The cherry juice and nanopure water solution was calculated by using the linear regression equation seen in Figure 1. The cherry juice concentration and nanopure was 2.26 uM. The stock solution was calculated by using m1v1=m2v2. The cherry juice concentra-tion and nanopure water mass, uM, was m1 and the 25 mL of the diluted solution was v1. The v2 was the 0.50 mL of the cherry juice. After calculations, the m2 was calculated. It ended up being 131 µM which was the mass of the stock
The concentration of the stock solution was found on the label and recorded in the lab notebook. It was labeled in the notebook as solution #1. 60.00 mL was removed from the solution using a volumetric pipet and was placed in a 100 mL volumetric flask. The solution was diluted to the mark on the flask. It was then covered with a stopper and mixed gently. The flask was labeled solution
Vitamin C helps to produce collagen. It is also vital in the production of dopamine and adrenaline, and necessary to the function of almost all major organs. Low levels of ascorbic acid can result in loose teeth, anemia, inability to heal quickly, and easier bruising. In general, though, people with fairly healthy diets get enough of this
My dependant variable is the amount of Vitamin C in each fruit juice. I will observe the solution when it is decolourizing. I will use the same volume of DCPIP solution for each fruit juice so that the results are accurate and valid. I will use the results from this experiment to work out how much vitamin C the fruit juices contain.
| Different concentrations of sucrose solution (specifically, 0.25mol/dm³, 0.5mol/dm³, 0.75mol/dm³and 1.00ml/dm³) and distilled water (0.00mol/dm³) will be used.
Vitamin C is an organic compound that is also known as ascorbic acid. The molecular formula for Vitamin C is C6O8H6. This structure contains a 5-membered ring. Vitamin C contains 4 alcohol functional groups, an alkene, and an ester. Within the ring it contains one double bond, the carbon=carbon bond in the ring structure, this is an alkene functional group. The Vitamin C structure also contains an ester which helps form the five-membered ring.
Vitamin C should be taken in through diets, fruits and vegetables so that it can have its best effects and is good for our health. Vitamin C supplements are only used as addition if our bodies do not have enough Vitamin C.
This essential vitamin can be found in diets consisting of citrus fruits, green vegetables, and potatoes. As excellent sources of vitamins, one serving of these foods contain at least ten percent of the recommended daily allowance. An international minimum standard for Vitamin C is ninety mg per day for a male adult, seventy-five mg per day for a female adult, and one hundred to one hundred twenty mg per day for a nursing female.
Vitamin C is crucial for survival for several reasons. For a human body to reach the acme of its physical potential, it must have a daily intake of vitamin C. Deficiency can lead to a fatal symptom known as scurvy. Likewise, iron absorption will be more difficult resulting in iron-deficiency anemia (Smith, Primio & Humphrey-Murto, 2011). However, this nutrient is essential for bodily functions as well. Vitamin C is required for hormone production which is seen by the fact that the highest concentration of it in the human body is in the pituitary and adrenal glands (Emsley, 2004). Moreover, this vitamin aids in protection against free radicals and stress. Vitamin C is also imperative because it assists in the growth of various body parts, including teeth, cartilage, and dentine. This acid is integral for bone development and to heal fractured or broken bones. As said before, “Vitamin C is important in the absorption and metabolism of many nutrients that affect production of red blood cells”, including iron absorption (Smith, Primio, & Humphrey-Murto, 2011). DIRECT QUOTE WITHOUT AUTHOR. Similar to its importance to humans, vitamin C is vital for plant life as well. Plants require the alcohol for growth processes.
Vitamin C, also known as ascorbic acid C6H8O6, is important because the body uses it in order to grow and repair tissues.1 Humans are unable synthesize vitamin C, therefore it is essential to consume the recommended daily amount around 60 mg/day.2 The goals of this lab were to determine the amount of vitamin C in a sample of apple juice. Also, another objective was to gain experience with redox reactions and titrations. “A titration is a method of analysis that determines the precise endpoint of a reaction and therefore the precise quantity of reactant in the titration flask”.3 Titrations are important because they provide accurate information on how to find the unknown concentration of a solution. Consequently, it is crucial that titration techniques are precise so the information is accurate. The information is calculated using the chemical formula.