Vitamin C

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An experiment to investigate the enzyme concentration and rate of reaction Background To carry out our experiment we used 3 different types of orange juices, and we also used 0.1% of vitamin C solution. The aim of our experiment was to see how much DCPIP was needed to be added to make the orange juices turn back to the orange colour and the DCPIP to decolourise once the 0.1% of vitamin C was added. DCPIP is a blue dye in its non-reduced form; it becomes colourless when it gains electrons. Vitamin C is an anti – oxidant, this is found mainly in fresh fruit and vegetables. The main use of Vitamin C is that it neutralises free radicals, which can cause damage to cells, including cells in the cardiovascular system Planning My aim in this…show more content…
| We did not decolourise the DCPIP, when adding the orange juices. As we had done for the vitamin C solution | 5 | Repeat this procedure with the fruit juices provided. If only one or two drops of the fruit juices decolourises the DCPIP, dilute the juice and repeat the test. | To obtain an average to measure whether our results match the fact that there is meant to be 100mg of vitamin C in the juices. | At this point we would get the result of the vitamin C and one of the juice means and divide them two and times it by a 100 to see how are results are, if it is close to the actual number it will mean it is reliable and accurate, however if it is not near the number it means that it is not very accurate. | Limitation of the apparatus and method Throughout the experiment I felt that there were many things that could have been improved on, so if I were to do the investigation with the modifications, I would get the correct and accurate results. Firstly the syringes that we were using to measure the liquids were not very accurate because, it did not have the lines between the whole numbers, therefore forcing us to guess the amount that was needed. A more precise piece of equipment would be needed instead such as a burette, if this experiment was done on a larger scale this would be more efficient, and accurate. Another limitation with the experiment was that there was not enough DCPIP, thus limiting the amount of repeats being

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