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Vj Junction

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1.) It can be assured that the heavy chain will create a VDJ junction but not a VJ junction because of the 12/23 rule. A gene segment flanked by an RSS with a 12 base pair spacer typically can only be joined to one flanked by a 23 base pair spacer RSS. So, for the heavy chain, a D segment can be joined to a J gene segment and a V gene segment to D gene segment but the V gene segment can not be directly joined to the J gene segment. Both the V and J gene segments are flanked by a 23 base pair spacer and the D segments have 12 base pair spacers on both sides. The light chain VJ junction will always be derived from a single type of light chain and not the result of κ or λ hybridization because the κ and λ are two separate loci that are located on two different chromosomes.…show more content…
The κ loci is located on human chromosome 2, the cluster of V gene segments is followed by a cluster of J gene segments and then a single C gene. The light chain VJ segment cannot be a product of κ or λ hybridization because in order for that to occur, they would have to be located on the same chromosome (Murphy 2012). Production of a complete antigen receptor includes two series of gene segment rearrangements, one for each receptor chain locus. Each of these rearrangements continues until a complete protein is made and the cell moves on to the next stage in development. The most important factor is the V regions that are encoded by separate gene segments (V, D and J segments), which are brought together by somatic recombination to create a complete V region exon. The V region of an immunoglobulin heavy or light chain is encoded by multiple gene segments. For the light chain, the V domain is made up of 2 gene segments, the V gene segment and the J gene segment. The joining of the V and J segments creates an exon that encodes the entire light chain V
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