Walter Groundcherry

From September 11, 1924 (Moritz 270), through the late 1960’s, Tom Landry accomplished a lot of things in his life, and set new trends for many years to come. Whether it be high school football star, or flying combat missions with the United States Air Force. Whether it be playing collegiate football, and really excelling, or actually moving on to a professional football career. Whether it be transforming from player to coach, and leading way for years to come and different programs; Tom Landry had experienced it all. Tom Landry was a great coach and player, whose leadership made him a hero, and a prestigious name in the football world.

A possibility of not getting a fragment under the test food with plant primers slot could be due to the fact of not extracting enough DNA from the sample food. Another reason for any missing fragments could be that the DNA ran off the gel, or that cross-contamination occurred with improper labeling of PCR tubes. These errors could be avoided by easily using the right sanitation methods, following the protocol and ensuring the slurry mix contained a good ratio of sample food to

The painting of Prof. Felix Walter (Figure 1) made with watercolour on paper on stretcher by Charles Fraser Comfort stands out as an intriguing piece of artwork because of the visual choices C. F. Comfort has produced. I was able to appreciate this work as I had the privilege of attending the Nuit Blanche exhibit at the Winnipeg Art Gallery, Winnipeg on the first of October, 2016 where Prof. Felix Walter was in display at. I found this particular artistry stood out because of its placement in dominance from the other paintings, the use of contrasting styles using the same medium and the bold textures that divulge the prominence of the subject. The artist, C. F. Comfort is worth being aware of because of his exceptional work such as this painting. The composition fashioned by the art gallery should also be duly noted as it took part in making this particular piece stand out.

PCR, also known as polymerase chain reaction is used

They were run at 1X 94ºC for 3 minutes, 30X at 94ºC for 30 seconds; 50ºC for 30 seconds; 72ºC for 45 second and 1X at 72ºC for 5 minutes. The PCR reactions took about 1 hour and 30 minutes to complete. The PCR products, were then purified by removing the leftover primers, nucleotides and salts. 250 µl of Buffer BB were added to Tube B and the mixture was pipetted into a spin column. The mixture was centrifuged for 30 seconds at room temperature. Then 2 cycles were completed at 30 seconds each with 200 µl of Buffer WB to remove any impurities. Then 25µl of Buffer EB were added to the tube to release the pure DNA and the mixture was centrifuged for 30 seconds. As the PCR reaction was running, a microscope slide was prepared from the live bacterial culture to observe the individual cells of the unknown bacteria and determine its

Our materials for the extraction of DNA included: the screwcap tubes, capless tubes, pipet tips, disposable transfer pipets, a foam microtube holder, a DI water bottle, a sharpie to label, a hot water bath, Non-GMO food, Test food, and a centrifuge. Our materials for the setup of the PCR reactions included: PCR tubes, screwcap tubes, pipet tips, an aerosol barrier, a foam microtube holder, a PCR tube holder, a 2-20 microliter micropipette, an ice bath, a sharpie to label, a plastic bag, a styrofoam cup with ice water, and a black tray with ice water. Finally, the materials for the electrophoresis of the PCR products included: Agarose gel, PCR sample tubes from previous labs, PCR tube holders, running buffers (300-350 ml), loading dye, a PCR molecular weight

Unfortunately, no raw values obtained for GAPDH proteins. On the other hand, PTEN protein shows relatively high raw values as compare to the representative data indicating PTEN expression does not affected by siGAPDH.

The Blue sample, which was hypothesized to be recombinant, should have produced similar if not identical bands to the known recombinant Green sample, which is not the case in the PCR gel. Instead, the Blue sample shows faint bands more closely resembling the Red sample, known to be non-recombinant. The bands did not show through for the PCR samples which indicates that the PCR preparation was not successful. This is further confirmed when analyzing the positive control which produced no results. The lack of bands in the negative control suggest that failure this portion of the experiment was not due to

In the year 1882 a man by the name of Walter Flemmings found out the process of mitosis in greater depth. A German zoologist Otto Bütschli might have discovered a process known as "mitosis", nevertheless he wasn't able to explain the topic in understandable vocabulary. Originally mitosis was discovered in frog, rabbit, and cat cornea cells and described briefly by the Polish histologist Wacław Mayzel in 1875. Flemmings got the fame because of his explanation. Now most of you might not know what this is since it is fairly new. This is a process where body cells or somatic cells are produced concluding the end product to be identical to one another. This an asexual process of cell division. In other words mitosis is a part of the cell cycle

As for Figure 2, it represents the electrophoresis that was obtained after the PCR amplification took place. When interpreting it one may notice that the band appears slightly low. This may indicate that the sample has a lower molecular weight, therefore appearing lower on the gel. This also helps support the hypothesis because the target sequence has 25 base pairs, which would also have a low molecular weight, so it will also appear lower in the gel.

With the sample wells near the cathode of the electrophoresis chamber, a potential difference of 100 volts was applied across the gel. The chamber was covered with aluminum foil to protect the SYBR SafeTM dye from light. Once the Orange G reached about 80% across the gel, the electrophoresis was stopped and the gel was carefully removed. In order to visualize the results of the PCR and electrophoresis, an image of the gel was taken under ultraviolet light. The resulting bands of the control samples were verified. Using the bands of the control samples as a guide, the presence of the Bt gene was evaluated by comparing the bands produced by the test corn chip.

Polymerase Chain Reaction (PCR) is a laboratory technique commonly used to make many copies of a region of DNA. PCR has many research and practical applications and It is routinely

Overall, the results suggest that, this was somewhat of an effective method in isolation the enzyme GAPDH from yeast

Same size of bands observed in clone #1-#3, it suggests that the PCR amplicons were obtained and indeed these were the mutant fragment.1

aPlant Molecular Biology Laboratory, Department of Botany, Faculty of Science, Dayalbagh Educational Institute (Deemed University), Dayalbagh, Agra-282005, India, Tel: 0562-2801545, Fax: 0562-2801226