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What Is Affinity Chromatography

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Affinity chromatography can also be used to isolate specific antibodies or antigens from antiserum. Antigen is covalently bound to small, chemically non-reactive beads and loaded onto a column. Antiserum is passed over the beads which results in binding of antibodies to the antigen. Specific antibodies are then eluted by normally lowering the pH to 2.5 or raising it to >11. Similarly antigens can be purified by binding specific antibodies to the
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