What Is Zero Caiso ( Water Enhancer?

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MIO (water enhancer) and a variety of other brands are labeled as “zero calorie”, and “all natural”. Not much research has been provided to validate this advertisement. People purchase water enhancers for benefits including weight loss and convenience. It is expected that MIO will show a significant increase in heart rate and the recovery rate after consuming. MIO is known to have additives like “Acesulfame Potassium” as a zero calorie sweetener. Acesulfame Potassium is mixed in with sucralose, and is up to 200 times sweeter than sugar. Acesulfame Potassium contains the carcinogen methylene chloride, exposure to this chemical over time can cause depression, mental confusion, liver and kidney complications, visual
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Contractions propel the blood from the posterior dorsal end of the worm. Pulsation waves are easily spotted as the outer layer of the worm is transparent, and contraction waves are seen in the main vessel as a heart beat. (Lesiuk and Drewes, 1999b) Pulsation rate is sensitive to pharmacological agents and is significantly accelerated by both caffeine and nicotine. (Bohrer, K.E. 2006) Much like in humans, the pulsation rate is regulated by the nervous and endocrine systems. Blackworms also have a very permeable outer skin layer, making the absorption of chemicals very efficient. Most consumers don’t think twice about the chemicals they are drinking, making most companies soar through the market as they label themselves as a “healthy” drink. MIO, a widely known company that produces water enhancers that are zero calories, do have many harmful chemicals and dyes. Many of which could lead to depression, hypersensitivity, cancer, and liver and kidney problems. This experiment was concluded to measure the heart and the recovery rate of blackworms (Lumbriculus variegatus), for relation to humans that consume zero calorie drinks like MIO. This experiment tests the effects of MIO on the circulatory system of lumbriculus variegatus, and the recovery rate after exposure to the water enhancer. An LD 50 test was also conducted.

Heart rate
All materials were obtained to start the experiment. To measure the heart rate after one minute of exposure, 44
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