What Protective Effect Of Α -lipoic Acid ( La ) Against Oxidative Stress And Apoptosis

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Increased oxidative stress and apoptosis have been implicated in Methotrexate (MTX)- induced oral mucositis that limits its clinical use. The present study was conducted to investigate the conceivable protective effect of α-lipoic acid (LA) against MTX induced toxicity on both buccal and lingual mucosae. Thirty adult male albino rats were divided into three groups; control treated with normal saline, MTX-treated group subjected to single intraperitoneal injection of MTX (20 mg/kg, i.p.) and LA treated group treated with daily intraperitoneal injection of LA (10 mg/kg, i.p.) for 5 weeks before MTX injection (20 mg/kg, i.p.). Rats were then sacrificed under anesthesia then their buccal and lingual mucosae were dissected out and processed for biochemical and histopathological studies. Malondialdehyde (MDA), reduced glutathione (GSH) contents, catalase, superoxide dismutase activity (SOD), integrity of nuclear DNA (nDNA) were estimated. Immunostaining was used to determine Bax and PCNA localization. MTX-treated rats showed increased levels of MDA and fragmentation of DNA in addition to reduction of GSH levels and activities of catalase and SOD. Histological examination of MTX-treated rats demonstrated degenerative changes that involved the surface epithelium and lamina propria of their buccal and lingual mucosae. Immunohistochemical results of MTX-treated rats revealed strongly positive Bax and weakly positive PCNA staining reactivity of the nuclei of the basal and

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