What are Aseptic Techniques?

Disinfection is used when a piece of equipment may have been contamination with a pathogenic micro-organism from mucous membranes or bodily fluids. Disinfection reduces the amount of micro-organisms but it will not destroy all bacteria or viruses. There are two methods of disinfection Heat and chemical disinfectants. Auto-claves are used in the hospital for items e.g. bed pans, endoscopes and devices used on a

Sterile Processing compromises a service of which the medical and surgical supplies of any medical facility’s equipment and instrumentation- sterile or non-sterile are cleaned, prepared, processed, stored, and distributed throughout the healthcare facility. There are several functions of this highly skilled team perform on a daily basis from decontamination to the sterilization of high risk medical implant devices. They are the first line of defense in infection prevention for any patient receiving medical treatment in healthcare facilities. Japp (2014) affirmed the importance of properly processing medical devices performed by Sterile Processing technicians for patient safety by stating a critical point “Not following the correct process can be detrimental to the patient as the device that we are assuming is safe for the patient may all actually cause severe illness or death” (p.

9. After the 48 hour time period the inhibition of the Escherichia coli bacteria around the disinfectant disks was measured. A ruler was used to measure the clearing from the edge of the disk to the perimeter of the clearing. Irregularities in the clearing were not included in the area of clearing.

The last form of sterilization I am going to talk about is cold sterile. The reason for cold sterile is not all items can go into heat sterilization. Liquid sterilant, such as 2% to 3.4% glutataldehyde, must be used for this type of sterilization. In order for the cold sterile to work the items must be fully submerged for no less than 10 hours, anything less than that would only be disinfection. This form of sterilization also requires a material safety data sheet due to it being a chemical.

This experiment illustrates the importance of handwashing and proves that hand washing is worth it. Since our hands are constantly coming into contact with ourselves and others, touching surfaces, grabbing objects, being sneezed into, etc., keeping our hands clean is one of the most effective, yet simple way we can take to avoid getting sick and spreading germs to others. Many diseases and conditions are spread by not washing hands with soap and clean, running warm water. “The human skin is a host to anywhere between 10,000-10,000,000 bacteria per square centimeter and since health care providers come into contact with pathogenic bacteria by being engaged in patient care, hand washing can reduce the risk of spreading diseases (page 3).” The objective of the experiment is to test the effectiveness of hand washing and demonstrate normal flora. This report presents the procedures and materials for the experiment, the experiment's results, and an analysis of those results.

A) Heat is an effective means of sterilization because it destroys the proteins of microbial

Health Care providers must implement infection control at all times. This is essential in order to avoid any sources of contamination that would put patients at risk for infection as well as all hospital personnel. There are two important aseptic techniques: Medical Asepsis, and Surgical Asepsis. The first one includes hand hygiene, use of gloves, masks, gowns and routine

The test tube was labeled with the bacteria identifying number. The cap on test tube was removed, and the lip of the test tube was flamed. Next, the Bunsen burner was used to sterilize the inoculating loop. Then, bacteria were picked up from the working plate with the loop and the agar was inoculated. The loop was then re-flamed. Finally, the plate was placed in the 37˚C incubator and left to sit for 48 hours and any changes in color were observed. A negative result appears green, and a positive result appears blue. This is because it tests for the organism’s ability to use citrate as its sole source of carbon, and if it does then it produces ammonia and ammonium hydroxide which make the medium basic, changing the green agar blue (Leboffe & Pierce,

Making hand washing a habit. Wash hands with soap and water after potential contact with human feces, handling pets, after gardening or contact with soil, before and after food handling, before and after having intercourse. Use alcohol-based cleansers such as hand sanitizers or disinfectants may also be used but not recommended for C.diff spores. Be extra careful with drinking water especially when you go out of the country. Use of proper attire when traveling and going to the mountains to protect self from unwanted animal bites. Make sure that you eat meat that was cooked thoroughly and uncontaminated to avoid food poisoning. Also get vaccines

Testing the Effects of Bacteria Growth on a Non-Treated Vs. Treated Hand Name: Laura Torres Biology 1407-P Abstract The objective of this experiment was to test the effects of bacteria using a treated hand and an unwashed hand. Two plates of agar and a hand is used to retrieve the bacteria and then placed in a temperature where they can survive. Different types of bacteria are affected by the environment that slow down or increase the growth of these prokaryotes.

A simple touch to the forehead or placing your gloved hand inside your scrub pocket are surefire ways to transmit pathogens, unintentionally. Something as mindless as these actions can place serious risk to you and your patient. Patients in all sorts of healthcare settings are already at risk of becoming infected due to the nature of their illness, increases pathogen presence due to hospitalization, and invasive procedures. The best and most effective way to prevent the spread of infection is to wash hands and maintain aseptic or clean technique. Asepsis is the absence of disease causing microorganisms. Aseptic technique is divided into two categories: medical and surgical. Surgical asepsis is used in a surgical setting where invasive procedures

Being able to control bacterial growth is something that is important in our everyday lives. As shown in the previous labs, bacteria can grow and create colonies extremely quickly especially in the right environments. By acknowledging this, it is then important to get an understanding of how bacterial growth can be controlled by humans. To control microorganisms it means to inhibit their growth (static) and or kill them (cidal) (Kenneth Todar, 2015); therefore since focusing on bacteria the terms bactericidal and bacteriostatic are both extremely important for this lab. One broad method we will use to control bacterial growth is heat. The amount of heat needed to control bacterial growth is different for different species of bacteria (Kenneth Todar, 2015). Bacteria can also respond differently depending if moist heating method such as an autoclave with steam is used, or a dry heating method such as inoculating a loop over a fire is used (Kenneth Todar, 2015). UV works by damaging the cells DNA, without proper DNA, the cells will die and the object

Two petri dishes with glucose and nitrogen rich mediums were each inoculated with cells obtained from different locations within the Biology building using aseptic techniques: the sink handles located on the left side of the lab room and the lab floor. An additional two petri dishes with mediums were obtained in order to purposely engage in poor laboratory techniques. One petri dish was left open for approximately an hour and a half and the other petri dish was touched by each group member’s finger. The four petri dishes were placed in an incubator set at 37°C for a week to allow for sufficient bacterial growth (Holbrook and Leicht 2017).

The purpose of the two experiments was to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli, Pseudomonas fluorescens, Enterococcus faecalis, Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5, 25, 37, 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different species. After one week they were observed for distinguishable changes by the turbidity showing an indication of bacterial growth, or the clarity an indication of no survival.

A fresh pipette was used to transfer 0.5ml of broth culture of E. coli, to be inoculated, into a tube of molten agar previously boiled to drive off oxygen. The tube was then rolled to distribute the bacteria and allowed time for the agar to harden. The tube was incubated at 37oC for 48 hours. The procedure was repeated for broth cultures of the bacterium Clostridium sporogenes and B. Subtilis.