Whole Cell Bioluminescent Bacterial Biosensors

2391 WordsMar 23, 201510 Pages
Whole cell bioluminescent bacterial biosensors. Introduction A biosensor is a system or device that is used for the measurement of a particular substance in which a biological component is used as the recognition element. (Urban, 2013). Microbial biosensors are analytical devices that combine a biological sensing element with a transducer to produce a signal proportional to the analyte concentration. They utilise immobilized viable or non viable microbial cells. Viable whole cell biosensors incorporate inherent metabolic and respiratory functions of the cell and the substance or analyte that is to be measured interacts with this process in a manner that it either inhibits or is a substrate of these processes. (Turner, 2015). A wide range of biomolecules such as enzymes, microorganisms and receptors as well as either whole or attenuated animal or plant cells have been utilised as biological sensing elements. Microorganisms offer the advantage of ability to detect a wide range of chemical substances, can be easily genetically modified, and possess a broad operational pH and temperature range, making them ideal as biological sensing materials. (Nivens et al., 2004). Bioluminescent bacterial biosensors are of particular interest, bioluminescent bioreporters have become popular, because the bioluminescent response is easily detected and the assay need not be destructive to the cells. Thus, the bioreporter can be continuously monitored in real time. (Simpson et al., 1998).
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