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Why Is The Bacteria? Essay

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In order to identify the unknown #75, there are several key concepts that should be remembered to successfully identify the bacteria. Initially a broth of an unknown was given containing both positive and negative unknown bacteria. It is important to observe aseptic technique to successfully and accurately identify the unknown bacteria that was given. Aseptic technique is used to avoid contamination. This could vary from contamination from the air to contamination with the materials that are being used for the experiment. If contamination occurs, then the unknown could produce a false result. Bacteria is usually transferred using an inoculating loop or needle and before transferring the bacteria, the tools must be sterilized in an incinerator by exposing them to high heat. The high temperatures produced by the incinerator effectively kills any contamination that was present on the tools. (1)
Equally as important, the bacteria being transferred must not be contaminated. If the bacteria are in broth for example, the test tube must be flamed before and after transfer. By flaming the test tube before transfer it creates a vacuum that prevents contamination. It is important to observe aseptic technique while performing experiments, so that the chance of contaminating the original bacteria and the bacteria being transferred may be avoided. The exposure to air is enough to contaminate bacteria and it can lead to false results. (1) After proper isolation of colonies of a

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