In order to identify the unknown #75, there are several key concepts that should be remembered to successfully identify the bacteria. Initially a broth of an unknown was given containing both positive and negative unknown bacteria. It is important to observe aseptic technique to successfully and accurately identify the unknown bacteria that was given. Aseptic technique is used to avoid contamination. This could vary from contamination from the air to contamination with the materials that are being used for the experiment. If contamination occurs, then the unknown could produce a false result. Bacteria is usually transferred using an inoculating loop or needle and before transferring the bacteria, the tools must be sterilized in an incinerator by exposing them to high heat. The high temperatures produced by the incinerator effectively kills any contamination that was present on the tools. (1)
Equally as important, the bacteria being transferred must not be contaminated. If the bacteria are in broth for example, the test tube must be flamed before and after transfer. By flaming the test tube before transfer it creates a vacuum that prevents contamination. It is important to observe aseptic technique while performing experiments, so that the chance of contaminating the original bacteria and the bacteria being transferred may be avoided. The exposure to air is enough to contaminate bacteria and it can lead to false results. (1) After proper isolation of colonies of a
There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. The experiment was done by applying methods in order to identify an unknown bacterium.
Poor hand hygiene - spread of germs from one patient to another or spread for hands to surfaces.
Cleaning is the first step in reprocessing instruments and it is called decontamination. The cleaning process is all soiled instruments placed in the prewash ultrasonic washer for ten minutes to remove any bacterial spores for instance, blood, tissue, and bone fragments. Then the instruments are sent through a wall washer. The wall washers are designed for the final cleaning of all instruments. Once the washer is finished, it unloads in the
However, everybody who has contact with the patient or the environment is entitled to relevant information that will enable them to reduce the risks of transmission to
11. An important tool available in the Virtual Unknown program is the Identification Matrix. From the portion of the identification matrix shown in the Identifying Bacteria tutorial, identify at least one bacterium that has a positive result to the arabinose fermentation test. (1 pt)
For many years the identification of microorganisms has been important in the world of medicine. It is essential or correct disease diagnosis in patients and for proper treatment. Knowing the correct identity and characteristics of microorganism is crucial when disease outbreaks occur in populations, also knowing how humans can benefit from microorganisms is important; many can be used in making certain foods or antibiotics.
There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that I have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium.
This experiment was conducted to find the genus and species of an unknown bacteria prescribed by the lab teacher, which was unknown bacteria GA3 in my case. Identification of unknown bacteria techniques are used on an every day basis to figure out what type of bacteria it is and to find the best method of how to treat a patient with this bacteria (1). All five “I’s” of Microbiology were used in the testing for the unknown culture. Inoculation was used several times to put the unknown culture into agar plates or into biochemical test tubes. After Inoculation of these tubes or plates, they always were placed into the incubator for further growth and development. Isolation was used to make sure we got the correct bacteria we were testing for. After each further isolation, we gram stained the culture and inspected the culture under a microscope to further help in the identification process of the unknown bacteria. Multiple tests were done on the unknown culture to make sure we were confident in what kind of bacteria the unknown was.
The main idea of this experiment was to correctly identify the unknown bacteria, #3. Identification of unknown bacteria yields multiple benefits in many different areas in the research of microorganisms. In this experiment I performed many different test dealing with things such as the presence of enzymes, fermentation abilities and different chemical reactions. Observations made from the tests were then compared to a gram negative unknown chart in order to identify the bacteria. Based off of my results and the chart, I concluded the bacteria #3 was the bacteria Escherichia coli. E. coli is most commonly found in the intestines of warm blooded organisms. Most E. coli strands are non pathogenic however, there are strands
Two smears of the unknown bacterium #5 were inoculated while the second smear was used for a back up. The unknown bacterium dried for at least forty minutes. After the smears dried, the slides were heat fixed two times to ensure the stability of the organism. The slide was placed on top of the staining rack then over the small sink.
Controlling the growth of microorganisms is not always necessary, but when they become a danger to society there is a need. There are several effective ways that are used to either control or destroy the microorganism. Bactericidal refers to the killing of microorganisms and bacteriostatic refers to inhibiting the growth. Heat is most often used for killing microorganisms. Some examples of heat would be incineration which is used for needles, inoculating wires and glassware. Boiling is used for to kill endospores and sterilize a solution. Autoclaves use pressurized steam which can denature or destroy heat labile substances, but is good for sterilizing almost everything. A hot air oven ( dry heat) is used for glassware, metal and objects that do not melt. Hospitals use autoclaves to clean surgical instruments and anything that would be used for multiple patients. Some items are made for one time uses, such as for a patient that is isolation for an infectious microorganisms that
In class, we were given the task of identifying an unknown bacterium broth culture. After receiving number 69, I went through several tests to figure out what bacterium I received. First, I created a slide from my broth by putting a small amount of the unknown broth on to a clean slide and letting it dry for ten minutes. After this, I stained the slide by applying four reagents in order; crystal violet, grams iodine, decolorizer and safranin. From the stained slide, I discovered that this bacterium was gram-negative, which would determine the next couple of tests I would do to identify my unknown bacterium. I began by streaking for confluent growth from my broth culture onto a TSA plate. From the TSA plate, I aseptically transferred a loop
The Aseptic technique is one of a number of procedures that contributes to preventing Health Care Associated Infections (HCAI)
Medical asepsis is concerned with destroying pathogenic organisms once they exit the host. To elaborate, medical asepsis technique is a process in which specific measures and procedures are utilized in order to prevent the transmission of disease causing organisms from person to person, or to healthcare staff. General examples of medical asepsis include wearing gloves, sanitizing various surfaces, and wearing Personal Protective Equipment (PPE). A more specific example of medical asepsis would be performing a chemical sterilization of a used gastrointestinal endoscope. Chemically sterilizing an endoscope involves cleansing the external and internal components of the endoscope by interchanging between brushing the parts with a disinfectant solution
sore throat, and chest pain can occur. The blood fails to clot and patients may