Winogradsky Column Discription

1129 WordsOct 11, 20125 Pages
Winogradsky column description Introduction The experiment of Winogradsky column is to observe microbiology interaction of microbes using pond mud. The aim of this paper is to describe structural and chemical features in Winogradsky column and to show the acknowledgement of microbial cells in Winogradsky column. Materials of experiment Pond mud Calcium sulfate Calcium carbonate Shredded paper Pond water Cylinder Aluminum foil Methods 1. Prepare pond mud 2. Add calcium sulfate and calcium carbonate 3. Add piece of paper 4. Add pond water 5. Seal top of the column 6. Put beside window Process (sequence of the reaction) The less of oxygen makes oxygen enrichment at the top as well as anaerobic…show more content…
(Perry, et al., 2002) Layers of column (Up to bottom) Water layer (green to clear) Diatoms, cyanobacteria, algae Oxygen dominated mud (light brown) Aerobic sulfide oxidizing bacteria (Beggiatoa, thiobacillus thiothrix) Rust colored zone Photoheterotrophs (Rhodospirillum, Rhodopseudomonas) Purple/reddish zone Purple sulfur bacteria (Chromatium) Green zone Green sulfur bacteria (Chlorobium) Sulfate reducing bacteria (Desulfovibrio) An aerobic zone Anaerobic bacteria (Chrostridium) (Delia Castro Anderson and Rosalina V. Hairston 1999) Discussion (related to the questions) In the experiment, we put calcium carbonate, calcium sulfite and shredded paper. Shredded paper contains cellulose which enables the bacteria (Clostridium) to break it down by fermentation to gain energy. Calcium sulfite was added to make H2S gradient. The bacteria Desulfobrio use sulfite as electron receptor. Low sulfur bacteria and high sulfur bacteria make layers. Calcium carbonate was used as carbon source by sulfur bacteria. These chemical materials eventually makes cycles of them. Typically, column contains O2, H2S, CO2, ethanol, organic acids, H2SO4 and possibly other chemical compounds that consist of carbonate, sulfite and the other in pond mud. We sealed top of column and that makes O2 gradient. Desulfovibrio gains energy using organic molecules which is made by Clostridium (anaerobic bacteria) and

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