Yeast Fermentation Lab Report

All devices and equipment were cleaned and returned to their rightful places and the lab area used was also tidied up.

Fermentation is a metabolic pathway that produce ATP molecules under anaerobic conditions (only undergoes glycolysis), NAD+ is used directly in glycolysis to form ATP molecules, which is not as efficient as cellular respiration because only 2ATP molecules are formed during the glycolysis. One type of fermentation is alcohol fermentation, it produces pyruvate molecules made by glycolysis and the yeast will break it down to give off carbon dioxide, the reactant is glucose and the byproducts are ethanol and carbon dioxide. In this lab, the purpose is to measure whether the changes of

tube was placed inside, then another test tube with an equal amount of substance would be placed

To performed the experiment, a volumeter was set up to measure the net oxygen production under white light, then a second step was followed to measure oxygen consumption under dark conditions (oxygen production only happens in the presence of light and oxygen consumption in the presence of dark light) and finally, a third step consisted of recording the measure of the net oxygen production under the presence of green light.

Abstract: This lab’s purpose was to see how different levels of yeast, distilled water, and sugar interact to affect the level of carbon dioxide evolved in fermentation. In this experiment we had two sections. The first section tested four test tubes with varying levels of yeast, glucose and distilled water for evolved carbon dioxide levels. The tubes were timed for 20 minutes. The amounts of solution in the test tubes are noted in the methods section of this lab report. The second section of the lab used three test tubes and flowed the same procedure except added spices. The levels of ingredients are also in the methods section. The main goal of this experiment was to see the effects of yeast concentration.

twice. Place the pH probe in the beaker and record the pH in the data table. Drag the beaker to the red

In the first part of the enzyme lab, we mixed a substrate and an indicator with an enzyme. There was also a neutral buffer in each of the chemical mixtures. The neutral buffer regulated the pH to around 7. We got a color palette and once we mixed each together, we observed and saw a change in the color of the substance. The darker and more brown the substance got, the more oxygen produced by the reaction. Our results showed that amount of oxygen produced increased about 10% a minute until it sort of equilibrated at 4 minutes and didn’t change to the fifth minute mark. If we were to change anything we did in the experiment, we would make our comparisons to the chart more precise. Overall we thought it

Place the Elodea in the water, start the stopwatch and measure and record the amount of oxygen bubbles produced by the Elodea over a 5-minute period.

The leveling bulb must be leveled with the amount of water in the buret at all times. Timing should begin after 2 ml of oxygen has been produced and must be recorded at 2 ml intervals until 14 ml of oxygen has accumulated. After recording the time, the first part of the experiment is now complete.

The null hypothesis for the first experiment was that substrate concentration would have no effect on the reaction rate. It was hypothesized that the reaction rate would increase with rising substrate concentrations, until all active sites were bound. The null hypothesis for the second experiment was that temperature would not have an effect on reaction rates. It was hypothesized that until the enzyme is denatured, as temperature increased, so would the reaction rate.

Higher levels of solution should produce higher levels of product. The independent variable for the control group data and the experiment data is the yeast concentration. The dependent variable for the control group data and the experiment data is how much oxygen is produced. The Constant for the control group data and the experiment data is time and amount of hydrogen peroxide. The products of the experiment will increase if the levels of reactants increase. Denatured yeast may cause change in the reaction of the experiment. For all trials of the control group, the concentration of yeast is 6ml. For the experiment data, the yeast concentration varies from 8mL, 10 mL, 12 mL, 14 mL, and 16 mL. The temperature may cause change in the reaction of the combination of yeast and hydrogen peroxide

1. Lab reports are to be computer-generated and double-spaced. All sections of the report must

All living organisms need the energy to perform the basic life functions. Cells use a process called cellular respiration to obtain the energy needed. In cellular respiration, cells convert energy molecules like starch or glucose into a cellular energy called Adenosine triphosphate(ATP). There are two types of cellular respiration which include: Aerobic and Anaerobic respiration. In aerobic respiration, cells will break down glucose to release a maximum amount of ATP this takes place in the presence of oxygen. Aerobic also produces carbon dioxide and water as waste products and it takes place in the mitochondria. on the other hand, anaerobic respiration, a metabolic process, also produces energy and uses glucose, but it releases less energy and does not require the

Hand out from the Natural Science Establishment, six dialysis tubing, six plastic cups, distilled water, 0.2 M, 0.4 M, 0.6 M, 0.8 M, and 1.0 M samples of a sucrose solution, paper towels, and a scale.

7.When water bath is ready, put each test tube into the water bath. Wait 5 minutes.