Yeast Lab Report

There are many substances that can be manipulated and cause the rate of reaction in fermentation to either speed up or slow down. Substances that alter the rate of the reaction could be temperature of the water, the yeast concentration, pH, and the glucose concentration. In the experimental group of the experiment the amount of yeast concentration was manipulated. The objective of this experiment was to determine what factors affect the rate of the fermentation. To test this objective we changed the amount of yeast being used. A higher yeast concentration replaced the controlled yeast amount. A prediction made by my group was that higher amount of yeast would speed up the process of fermentation. Our null hypothesis is there will be no

Saccharomyces cerevisae, or baker's yeast, is an example of a model organism to further explore the mechanisms of the human body, and other organisms. Ultraviolet radiation has a damaging effect on human cells, and sunscreen is often used as a means of protection from the UV radiation. Cultures of yeast were irradiated with an ultraviolet light under different standards of SPF sunscreen protection. The purpose of the experiment was to study the effects of UV radiation on yeast cells. Each yeast plate had either a different level of SPF sunscreen as protection, or no protection and was irradiated for 45 seconds. After a week, the number of colonies on each plate was counted. The colonies of the positive control were transferred to MV plates to see if any growth appeared one week later. The results are inconclusive due to the high standard deviations, no significant trends in colony survival, low replication number, and what seems to be human error.

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

The students of Mr. Woodruff’s 9th grade Pre-Ap Biology class performed a lab on how different carbohydrates affect the cellular respiration of Saccharomyces cerevisiae or yeast. Yeast is an eukaryotic and unicellular organism. It is eukaryotic because it contains a nucleus that is surrounded by a membrane and its DNA is bound by proteins to form chromosomes (Dictionary.com,Eukaryotic). Yeast is unicellular because it consists of only one cell. Yeast is important to humans because it helps with the fermentation process and it helps scientists see how human genetics work , because humans and yeast are both eukaryotic (Volk).In this experiment yeast is used instead of other unicellular organisms because it is eukaryotic and can respire aerobically

There are four main types of produced in our body based on how soon the insulin starts working, when it reaches the peak, and how long it stays in or body. Each type responds to insulin in a different way so there is no one type that works best for everyone. Some types are mixed together in one bottle.

Higher levels of solution should produce higher levels of product. The independent variable for the control group data and the experiment data is the yeast concentration. The dependent variable for the control group data and the experiment data is how much oxygen is produced. The Constant for the control group data and the experiment data is time and amount of hydrogen peroxide. The products of the experiment will increase if the levels of reactants increase. Denatured yeast may cause change in the reaction of the experiment. For all trials of the control group, the concentration of yeast is 6ml. For the experiment data, the yeast concentration varies from 8mL, 10 mL, 12 mL, 14 mL, and 16 mL. The temperature may cause change in the reaction of the combination of yeast and hydrogen peroxide

In general, in the fermentation lab the more gas produced showed the level of fermentation, the food source that produced the most CO2 the fastest during the fermentation process was the sample of 0.2 M glucose at 37°C topping of at 9.2ml of CO2 produced in 40 minutes total (see Table 2). This sums up the most optimal conditions for cell fermentation was a simple monosaccharaide, glucose, and at temperature of around 37 C which is the temperature of the human body. The hypothesis was correct a simple sugar easily broken down and higher temperatures are optimal. These results showed that the simpler the sugar the better it is for breaking it down. Sucrose is a disaccharide and starch is a polysaccharide. The hardest to break down was starch. This is possible due to

Students conducted this experiment to study the fermentation that took place in yeast by observing the results of CO2 production in different test tubes. Glucose was readily available for the respiration of the yeast so CO2 was produced. In tube4 the evolution of CO2 was less than tube2 because of the grounded yeast. The presence of CO2 in tube5 was the least due to the addition of acetic acid in it because acetic acid in the fermentation led to a significant decrease in the concentration which shows that the acetic acid is the inhibitory form of the molecule. For tubes 1 and 3 respiration was not present because one of the essential factors was absent (Yeast in tube3 and glucose in tube1). Another factor affecting the cellular respiration

In conclusion, making the observations of the red and white colonies of Saccharomyces cerevisiae gave me the thought that the hypothesis that was the best fit for this experiment was “Natural selection is the primary process involved in the populations’ evolution”, because it is clear that this occurred after the yeast was put into the new environment, the petri dish, that there was a difference between the red and the white colonies. It was a clear observation that just looking at the petri dish and data, there was a significant increase in white yeast populations compared to the red yeast colonies. This could suggest a greater amount of reproduction from the white colonies, meaning that they must have had a better adaption to their new environment

All living organisms need the energy to perform the basic life functions. Cells use a process called cellular respiration to obtain the energy needed. In cellular respiration, cells convert energy molecules like starch or glucose into a cellular energy called Adenosine triphosphate(ATP). There are two types of cellular respiration which include: Aerobic and Anaerobic respiration. In aerobic respiration, cells will break down glucose to release a maximum amount of ATP this takes place in the presence of oxygen. Aerobic also produces carbon dioxide and water as waste products and it takes place in the mitochondria. on the other hand, anaerobic respiration, a metabolic process, also produces energy and uses glucose, but it releases less energy and does not require the

Aim To show if sunscreen protects against UV-induced damage in Yeast. Introduction The purpose of this experiment was to show if sunscreen could protect against UV-induced damage in Yeast. The Yeast used was already deficient in DNA repair. This was determined by observing colony growth on a YED agar plate, in the absence and presence of sunscreen lotion, after exposure to UV light for 3-5 minutes.

Our colons are populated by a large colony of bacteria, which assists in the final stages of digestion

For the purpose of this experiment a forward genetic screening method was used. Saccaromyces Cerevisiae was mutagenized with UV radiation. In order to test whether or not the procedure worked, the mutated genes were plated on a complete (rich) medium and again on minimal medium. All of the genes grew on the complete medium because the nutrients necessary for growth were present but not all grew on the minimal medium. Those, which did not grow on the minimal medium, were classified as auxotrophs. An auxotroph is a mutant organism that requires a particular additional nutrient that the normal strain, wild type, does not (Dictionary). In other

Abstract: Many people use enrichment to grow cultures for mutant colonies. In this specific experiment we selected to kill the non-auxotrophic cells and find any auxotrophs that did not die during an enrichment process. We

Hydrogen peroxide is a toxic byproduct of cellular functions. To maintain hydrogen peroxide levels the catalase enzyme deconstructs hydrogen peroxide and reconstructs the reactants into oxygen gas and water. The catalase enzyme is found inside cells of most plants and animals. Regulating the levels of hydrogen peroxide is crucial in homeostasis and analyzing it’s optimal conditions for performance is just as important. To understand the optimal environment for this enzyme, they are put into different environments based off protein activity (enzymes are proteins). Catalase samples will be put into different hydrogen peroxide environments based off pH and temperature. The more active the enzyme, the more oxygen and water it will produce. Enzyme activity can be seen through the release of oxygen in the hydrogen peroxide. Since oxygen cannot be accurately measured, the data will consist of the longevity of the reaction in different environments. If the pH is higher than 7, then the reaction rate will increase due to the ample amount of hydrogen ions in the hydrogen peroxide. However the pH level cannot be higher than 10 or else there will be too many hydrogen atoms in the peroxide for the enzyme to be able to deconstruct them. If the temperature is increased, then the reaction rate will increase due to the ample amount of energy and movement in the hydrogen peroxide and enzyme.