effect of pH on amylase activity Essay

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Biology Lab Report

Research Question:

Effects of pH on amylase activity

Introduction:

Amylase is an enzyme that is in human’s saliva as well as the pancreas. Enzymes are biological catalysts that speed up a chemical reaction. They break down complex molecules into simple ones. In this case, amylase converts starches (complex molecule) into simple sugars. That is why foods like potatoes for example, may taste sweet to us, because they contain starch. The optimum pH for pancreatic amylase is the pH of 7. In the experiment I have used buffer solutions with the pHs of 2.8, 4 and 6.5. I have also used iodine and starch. Normally, iodine is orange-yellow, however when you add starch to it, the solution will turn blue-black.
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We have timed each of the three experiments for 5 minutes to make sure that our results are correct. Thanks to the program, we got the transmittance and the absorbance, together with the graph portraying our results.

Results:

pH 2.8

Time Transmittance Absorbance
0 32.0843924713 0.493706179806
3 28.0234356689 0.552478621349
6 29.255019455 0.533799608742
9 30.3841529083 0.517352867013
12 31.3585209656 0.503644429139
15 32.2282588959 0.491763156242
18 32.9214334869 0.482521262709
21 33.5339556885 0.474515213874
24 33.5383152771 0.474458757002
27 34.6303922272 0.460542589561
30 35.0728904724 0.455028441005
33 35.609119873 0.448438760361
36 35.8946729279 0.444969999603
39 36.2521591949 0.440666121547
42 36.5791283417 0.436766647709
45 36.9344348145 0.432568542341
48 37.2679433441 0.428664573411
51 37.4902823639 0.42608128861
54 37.7801950073 0.422735804742
57 37.9938148499 0.420287097994
60 38.1987155151 0.417951240598
63 38.3556607056 0.416170531799
66 38.5627411652 0.413832102607
69 38.7545630646 0.411677155168
72 38.9616435242 0.409362731505
75 39.1164089203 0.407641022435
78 39.2973318481 0.405636935705
81 39.4847941589 0.403570121697
84 39.6286605835 0.401990606218
87 39.8052239227 0.400059928631
90 39.9142136383 0.398872422472
93 40.0602598572
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