lab 6 Essay

799 Words4 Pages
Taylor Hill
Lab 6: Enzymes

Purpose: The purpose of this lab is to test for enzyme activity, look at enzyme specificity, and how temperature affects enzyme activity.

Hypothesis: I believe that the room temperature activity will produce the most activity.

Materials: 3% hydrogen peroxide, a household blender, potatoes (3 medium size potatoes, that are peeled and quartered), tap water, 8 test tubes, 8 transfer pipets, sugar packets, salt, a small saucepan, a refrigerator with a freezer, stove, 4 clear plastic cups, tongs (to remove test tubes from boiling water), 4 coffee cups, cheesecloth to filter the potato extract, a wax pencil or Sharpie marker, a ruler, scale or balance, 250 ml graduated cylinder, and oven mitt or tongs.
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Pick up each test tube, tap the bottom/ swirl the contents and then using your ruler record in Table 1 the bubble height (if any) that is produced. Remember to include this table (with a strong title) in your lab report.

Experiment 2- Testing the Effect of Temperature on Enzyme Activity
Step 1: Label the test tubes 1, 2, 3 and 4, where test tube 1 represents the sample placed on the counter at room temperature, test tube 2 represents the tube placed in the refrigerator, test tube 3 represents the tube placed in the freezer, and test tube 4 represents the sample exposed to boiling water. You will expose catalase to each of these four conditions.
Step 2: Place a saucepan with tap water on a burner and bring to a boil.
Step 3: Use the thermometer to identify and record the temperature for room temperature, in your refrigerator, in your freezer, and then research the temperature of boiling water (do not take this temperature) and record them in Table 2.
Step 4: Place the test tubes into separate coffee cups to maintain the upward position. Add 2 mls of the catalase solution to each of the test tubes and then place tubes1, 2, and 3 in the conditions described above. For test tube 4, fill the coffee mug half full of boiling

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