pBlu lab

1164 WordsFeb 27, 20145 Pages
February 20th, 2014 Lab report 4 Abstract This pBlu lab had for purpose to present the changes of the strain of E. coli bacteria due to new genetic information being introduced into the cell. In this experiment we are freezing and heat shocking the E. Coli bacteria that is then forced to take the plasmid DNA. The E. coli then transforms the pBLu plasmid, which carries the genes coding for two identifiable phenotypes. After following the Carolina Biological steps our lab worked well and we able to see some colonies of bacteria on the plates. The x-gal plate showed a significant amount of bacteria to confirm that the pBlu plasmid took over the E. coli strain. Introduction This lab was meant to reveal the variations of different…show more content…
LB= Growth medium of Agar/Luria Broth (used in all experiments) AMP= Ampicillin present X-gal= galactose sugar Clear= no growth at all. The results of this experiment ended up being very conclusive and not positively or negatively surprising. The data and results collected we what we had expected more importantly the ones on the X-Gal plates where the colonies were above 100. Both the stains of E. Coli grew colonies when they were in the LB. When the ampicillin was present the cells did not grow even with the X-gal. The transformed cells on the other hand grew when the ampicillin was there. When the X-gal and AMP were present the transformed cells grew and had a blue tint, which is why we call it the pBlu lab. (duh) Discussion What was expected that the strains of E. coli that did not have a resistance to ampicillin would not grow. The transformed strain also changed to a blue color when the X-gal was present in plate. The transformed cells also grew because they were free of the ampicillin because they possessed the amp gene that they used as an shield against the ampicillin antibiotic. The transformed cell who turned blue did so because the gene converted the sugar to a blue color but also contained the amp gene to ensure that they grew even when the ampicillin was present. The growth of the colonies on the plates
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