redox titration Essay

We know that that the end point of the titration is reached when, after drop after careful drop of NaOH, the solution in the flask retains its pale pink color while swirling for about 30

Next, I poured distilled water just below the 250ml mark on the neck of the volumetric flask.

c) Record the amount of substance that dissolved – all, some, a little, or none.

Enter the precise mass in GRAMS of the potassium iodate used to prepare your primary standard solution.

Discard the solution in the appropriate container as directed to you by your lab instructor.

Again, label 7 1.5ml tubes 0 thru 6. Place 15μl of each serially diluted extract into its corresponding labeled tube. Next add 465μl of media into each tube. Then 60μl of Alamar blue in each tube. Finally add an additional 60μl of cells (adjusted to 10,000 cells/20 μl). Vortex each tube for 5 seconds. Now, take 3 different samples 190μl samples of concentration 0 and put it in Wells A2, B2, and C2. Repeat this step again by taking 3 more different 190μl samples of concentration 1 and putting it in wells A3, B3, C3. It should be noted that it is important to vortex each 1.5μl tube again be-fore putting it into the 96 well plate. Contin-ue this same procedure consecutively for the re-maining concentrations.

Prepare standard solution #1, Take 1 ml sub stock solution from the 100 ml beaker and then put into 25 ml volumetric flask with the help of 10ml graduate pipette.

Pour approximately 50 mL of room-temperature distilled water into the glass beaker by using the estimated volume on the beaker.

When filling up the burette it is important that a funnel is used, however as the solution reaches the 0 mark it is ideal that the funnel be removed and a pipette used instead to reach the 0 mark, this is to achieve greater precision. During the experiment, it is important to swirl the flask continuously with one hand

1. Fill up a 200ml beaker with tap water and then pour it into the designated jar

8.) Add one drop of iodine solution to wells C,F and I. Record any changes you observe. Caution - iodine solution is corrosive and poisonous. It can stain skin and clothing. Rinse any iodine spills with water.

Place 100 ml of distilled water in a 250-ml (or 400-ml) beaker. Add 1.26g of oxalic acid dihydrate (H2C2O4.2H2O) and 1 ml of concentrated ammonia. Stir the mixture until the solid has dissolved completely.

If you see a deep blue color add more zinc powder until there is no change in color of the solution in the test tube.

1) Pour 25 mL of the 1 M hydrochloric acid into the beaker and rinse the solid by swirling the acid around in the bottom of the beaker.

Add the same amount of Benedict’s solution in each tube and for the ones that need iodine use the same amount.