1) If direct stains work so well, why would we ever use indirect stains? Give two reasons why you might choose to use indirect stains instead of direct stains? 2) Assume that two days (dye A and dye B) were applied to bacterial smear. The cells were stained by dye A while the slide background was stained with dye B. Which stain had an anionic chromogen? Which stain had a cationic chromogen?

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Asked Sep 11, 2019
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1) If direct stains work so well, why would we ever use indirect stains? Give two reasons why you might choose to use indirect stains instead of direct stains? 

2) Assume that two days (dye A and dye B) were applied to bacterial smear. The cells were stained by dye A while the slide background was stained with dye B. Which stain had an anionic chromogen? Which stain had a cationic chromogen? 

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Hi! Thanks for your question. But as you have posted multiple questions, we are answering only the first one for you. If you need help with the other questions too, please repost them separately.

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In negative or indirect staining, an acidic dye is required. The negatively charged color portion of the acidic dye is repelled by the negatively charged bacterial cell. Thus, as a result, the background bec...

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