1.) Describe the components that are important to a cloning vector.
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Q: What are the features of a plasmid being used as a cloning vector?
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Q: A. Please briefly explain how Polymerase Chain Reaction works to amplify DNA.
A: As per our policy, We are answering only question 1. For the rest of the questions please repost.…
Q: Restriction enzymes and DNA ligase play essential roles in DNA cloning. How is it that a bacterium…
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Q: Plasmids are the only vectors currently available for use in recombinant procedures.True or false?
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Q: How is a gene inserted into a plasmid cloning vector?
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Q: What would happen if a plasmid without a selectable marker was chosen as a cloning vector?
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Q: Create recombinant DNA molecules with plasmid vectors?
A: The DNA (deoxyribonucleic acid) is the genetic material of an organism that is inherited by the…
Q: How to create recombinant DNA molecules withplasmid vectors?
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Q: Explain the process of Cloning DNA into smaller vectors ?
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Q: Using one of the options below, finish the following statement so that it is correct. Plasmids are…
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Q: What is the function of the Ti plasmid in the creation of transgenic organism? Explain your answer.
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Q: What characteristics of plasmids and bacteriophages make themgood cloning vectors?
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Q: What are the essential elements of a plasmid cloning vector? Discuss the importance of each element…
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Q: In DNA cloning, why do we need to check colonies for insert after transformation?
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Q: What characteristics of plasmids makes them good cloning vectors? Discuss.
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Q: distinguish between bacterial cells that obtained the plasmid and those that did not
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Q: PLease explain What characteristics of plasmids makes them good cloning vectors? Discuss
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Q: Identify mutant genes by plasmid library transformation?
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Q: List and explain the steps of DNA cloning using pUC8.
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Q: Why are antibiotic resistance genes usually included in plasmids used for genetic transformation?
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Plasmids are very important for recombinant DNA work.
1.) Describe the components that are important to a cloning vector.
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- From where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase B) they are cut out of plasmids using restriction endonucleases C) DNA primase is added to the sequencing reaction and synthesizes the primers D) biotechnology companies synthesize them using organic chemistryIn cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into bacterium?I) Transform bacteria with recombinant DNA molecule.II) Cut the plasmid DNA using restriction enzymes.III) Extract plasmid DNA from bacterial cells.IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.V) Use ligase to seal plasmid DNA to non-plasmid DNA A. III, II, IV, V, I B. I, II, IV, III, V C. III, IV, V, I, II D. II, III, V, IV, I
- In DNA cloning, why do we need to check colonies for insert after transformation?Recombinant DNA construction involvesa) Cleaving DNA with restriction endonuclease and joining with ligaseb) Cleaving DNA with ligase and joining with endonucleasec) Cleaving and joining DNA with restriction endonucleased) Cleaving DNA with restriction endonuclease and joining with polymeraseWhen E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as : A. Ligation. B. Transformation. C. Transfection. D. Digestion.
- Based on the picture, why plasmids are used as a vector for DNA Recombination? What other vectors can be used?What characteristics of plasmids makes them good cloning vectors? Discuss.Restriction enzymes and DNA ligase play essential roles in DNA cloning. How is it that a bacterium that produces a restriction enzyme does not cut its own DNA?
- What is a plasmid? What are the minimum 3 components that a recombinant plasmid used for genetic transformation should have?Construct a restriction map for Plasmid X to show the recognition sites for two commonly used restriction enzymes: EcoRI and BamHI. Plasmid X undigested Plasmid X digested with EcoRI Plasmid X digested with BamHI Plasmid X digested with EcoRI and BamHI 2200 bp 1000 bp 1200 bp 400 bp 1800 bp 200 bp 400 bp 600 bp 1000 bpUsing nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid.What are restriction length polymorphisms, and how are they used?