1. Prepare a fresh stock solution of 50 cm3of 0.2% ascorbic acid. 2. Prepare 5 standard solutions of ascorbic acid in volumetric flasks of 25 cm3. 3. To each volumetric flask add 0.5 cm3 of 0.5M acetate buffer and different volumes of 0.2% ascorbic acid to make 0.01 mM, 0.02 mM, 0.03 mM, 0.04 mM and 0.05 mM solutions. Dilute to the mark with distilled water.
1. Prepare a fresh stock solution of 50 cm3of 0.2% ascorbic acid. 2. Prepare 5 standard solutions of ascorbic acid in volumetric flasks of 25 cm3. 3. To each volumetric flask add 0.5 cm3 of 0.5M acetate buffer and different volumes of 0.2% ascorbic acid to make 0.01 mM, 0.02 mM, 0.03 mM, 0.04 mM and 0.05 mM solutions. Dilute to the mark with distilled water.
Chapter31: Introduction To Analytical Separations
Section: Chapter Questions
Problem 31.19QAP
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How do I prepare these solutions? I do not know what volumes or molarities to be added from the initial step. Thank you.
1. Prepare a fresh stock solution of 50 cm3of 0.2% ascorbic acid.
2. Prepare 5 standard solutions of ascorbic acid in volumetric flasks of 25 cm3.
3. To each volumetric flask add 0.5 cm3 of 0.5M acetate buffer and different volumes of 0.2% ascorbic acid to make 0.01 mM, 0.02 mM, 0.03 mM, 0.04 mM and 0.05 mM solutions. Dilute to the mark with distilled water.
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