1. The nontemplate strand of a segment of double- helical DNA contains the sequence: (5')CGCTATAGCGTTTAG (3') (a) What is the MRNA base sequence that can be transcribed from this double helical DNA assuming you already have a ready RNA polymerase present and everything else needed for RNA transcription? Label the sequence ends and list what is required for RNA Pol to work. (b) What amino acid sequence could be coded by the mRNA in (a)'s answer assuming the AUG start codon is just upstream of the mRNA (not shown in the sequence provided)? Use table in Figure 27-7 of the Lehninger 7 th ed "Dictionary of amino acid code words in mRNAs" and write the peptide according to standard convention with abbreviations for the amino acids. (c) If the wrong strand was transcribed and then translated from the section of double stranded DNA shown above, what would the resulting amino acid sequence be? What would the MRNA sequence be? Explain your answer and label all strands. 系: 2222 阳 高的 the twentieth century First letter of codon (5' end) Second letter of codon U A UUU Phe UCU Ser u UUC Phe UCC Ser UAC Tyr UGC Cys UUA Leu UAU Tyr UGU Cys UUG Leu CUU Leu CAU His CGU Arg c| CÚC Leu CUA Leu CUG Leu CCA Pro CCG Pro ACU Thr ACC Thr ACA Thr ACG Thr AUU lle AUC lle AUA lle AUG Met AAG Lys GCU Ala GAU Asp GCC Ala GAC Asp GCA Ala GAA Glu GAG Glu GUU Val GUC Val GUA Val GUG Val GCG Ala

Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter15: Genes And Proteins
Section: Chapter Questions
Problem 29CTQ: A normal mRNA that reads 5’ - UGCCAUGGUAAUAACACAUGAGGCCUGAAC- 3’ has an insertion mutation that...
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1. The nontemplate strand of a segment of double-
helical DNA contains the sequence:
(5')CGCTATAGCGTTTAG (3') (a) What is the MRNA
base sequence that can be transcribed from this
double helical DNA assuming you already have a ready
RNA polymerase present and everything else needed
for RNA transcription? Label the sequence ends and list
what is required for RNA Pol to work. (b) What amino
acid sequence could be coded by the mRNA in (a)'s
answer assuming the AUG start codon is just upstream
of the MRNA (not shown in the sequence provided)?
Use table in Figure 27-7 of the Lehninger 7 th ed
"Dictionary of amino acid code words in mRNAs" and
write the peptide according to standard convention
with abbreviations for the amino acids. (c) If the wrong
strand was transcribed and then translated from the
section of double stranded DNA shown above, what
would the resulting amino acid sequence be? What
would the MRNA sequence be? Explain your answer
and label all strands.
歐歐队
相阳阳一即即的 9
First letter of codon (5' end)
Second letter
of codon
U
A
UUU Phe UCU Ser
u UUC Phe UCC Ser UAC Tyr
UAU Tyr
UGU Cys
UGC Cys
UUA Leu UCA Ser UAA Stop UGA Stop
UCG Ser
UUG Leu
UAG Stop
UGG Trp
CUU Leu
CCU Pro
CAU His
CCC Pro CAC His
CCA Pro CAA Gl
CAG Glr
c CUC Leu
CUG Leu
AUU lle
A AUC lle
AUA lle
AUG Met
ACU Thr
ACC The
AAA Lys
ACG Thr
AAG Lys
GAU Asp
GGU Glv
GCU Ala
GCC Ala
GCA Ala
GCG Ala
GAC Asp
GAA Glu
GAG Glu
GGA Gly
GGG Gly
Transcribed Image Text:1. The nontemplate strand of a segment of double- helical DNA contains the sequence: (5')CGCTATAGCGTTTAG (3') (a) What is the MRNA base sequence that can be transcribed from this double helical DNA assuming you already have a ready RNA polymerase present and everything else needed for RNA transcription? Label the sequence ends and list what is required for RNA Pol to work. (b) What amino acid sequence could be coded by the mRNA in (a)'s answer assuming the AUG start codon is just upstream of the MRNA (not shown in the sequence provided)? Use table in Figure 27-7 of the Lehninger 7 th ed "Dictionary of amino acid code words in mRNAs" and write the peptide according to standard convention with abbreviations for the amino acids. (c) If the wrong strand was transcribed and then translated from the section of double stranded DNA shown above, what would the resulting amino acid sequence be? What would the MRNA sequence be? Explain your answer and label all strands. 歐歐队 相阳阳一即即的 9 First letter of codon (5' end) Second letter of codon U A UUU Phe UCU Ser u UUC Phe UCC Ser UAC Tyr UAU Tyr UGU Cys UGC Cys UUA Leu UCA Ser UAA Stop UGA Stop UCG Ser UUG Leu UAG Stop UGG Trp CUU Leu CCU Pro CAU His CCC Pro CAC His CCA Pro CAA Gl CAG Glr c CUC Leu CUG Leu AUU lle A AUC lle AUA lle AUG Met ACU Thr ACC The AAA Lys ACG Thr AAG Lys GAU Asp GGU Glv GCU Ala GCC Ala GCA Ala GCG Ala GAC Asp GAA Glu GAG Glu GGA Gly GGG Gly
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