1. What is the purpose of flaming an inoculating loop? 2. How will you know when you have flamed the loop or needle long enough?  3. Why is it necessary to cool the inoculating loop prior to obtaining the bacterial sample? 4. In which direction should you move the inoculating loop in the Bunsen burner flame? (i.e. from the handle to the loop or from the loop to the handle)

Question
Asked Aug 28, 2019
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1. What is the purpose of flaming an inoculating loop? 

2. How will you know when you have flamed the loop or needle long enough?  

3. Why is it necessary to cool the inoculating loop prior to obtaining the bacterial sample? 

4. In which direction should you move the inoculating loop in the Bunsen burner flame? (i.e. from the handle to the loop or from the loop to the handle)

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Expert Answer

Step 1

Hi, since you have posted multiple questions, we will be providing you with answers for the first three questions. If you want any other specific question to be answered please submit them again separately. Thank you.

Step 2

1.

An inoculation loop is simple laboratory equipment mainly used by mycologists to transfer microorganisms to a growth media. The purpose of flaming an inoculating loop is to prevent or reduce the chances of cross-contamination of the cultures being inoculated.

Step 3

2.

It is required to sterilize the inoculating loop or needle by passing it an angle through the flame of a Bunsen burner until the whole length has turned into bright orange or red...

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