1. Which following statement is NOT correct for Matrix-Assisted Laser Desorption/Ionization (MALDI) MS technique? A. Low concentration of analyte uniformly dispensed in a solid or liquid matrix deposited on metal plate B.MALDI matrix must strongly absorb laser radiation C.Matrix materials is not applied for biomolecules with laser wavelengths D. Analyte concentration in μM range   2. The Beer-Lambert Law A states that transmittance is linearly related to concentration B. requires that the product of absorptivity and pathlength be known before absorbance can be related to concentration C.requires that absorptivity be independent of wavelength D. cannot be used if more than one absorbing species is present in a sample   3. Flame atomizers A. have an optical path length based on the length of the burner slot B. vary in temperature based on the choice of fuel and oxidant C. typically allow sample introduction by aspiration and nebulization of a liquid D. all of the above   4. Which figure of merit is a measure of instrument ability to discriminate between small differences in analyte concentration? A. Selectivity B.Precision C. Sensitivity D. Bias   5. Which noise below does NOT belong to instrument noise? A. Thermal noise B. Flicker noise C. Shot noise D. Vibrational noise   6. For sample containers in optical instruments, which statement is correct? A. Required for all spectroscopic studies B. Cell or cuvette made of material that is transparent to radiation in the spectral region of interest C. Plastic containers cannot be used for any spectrum D. Crystalline sodium chloride for cell windows in UV   7. Which statement for photon is wrong? A. Particles with electromagnetic radiation as a stream of discrete particles or wave packets of energy B. Energy is proportional to radiation frequency C.Possess wave-particle duality D. Behavior is completely explained by classical mechanics

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter13: An Introduction To Ultraviolet-visible Molecular Absorption Spectrometry
Section: Chapter Questions
Problem 13.20QAP
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1. Which following statement is NOT correct for Matrix-Assisted Laser Desorption/Ionization (MALDI) MS technique?

A. Low concentration of analyte uniformly dispensed in a solid or liquid matrix deposited on metal plate

B.MALDI matrix must strongly absorb laser radiation

C.Matrix materials is not applied for biomolecules with laser wavelengths

D. Analyte concentration in μM range

 

2. The Beer-Lambert Law

A states that transmittance is linearly related to concentration

B. requires that the product of absorptivity and pathlength be known before absorbance can be related to concentration

C.requires that absorptivity be independent of wavelength

D. cannot be used if more than one absorbing species is present in a sample

 

3. Flame atomizers

A. have an optical path length based on the length of the burner slot

B. vary in temperature based on the choice of fuel and oxidant

C. typically allow sample introduction by aspiration and nebulization of a liquid

D. all of the above

 

4. Which figure of merit is a measure of instrument ability to discriminate between small differences in analyte concentration?

A. Selectivity

B.Precision

C. Sensitivity

D. Bias

 

5. Which noise below does NOT belong to instrument noise?

A. Thermal noise

B. Flicker noise

C. Shot noise

D. Vibrational noise

 

6. For sample containers in optical instruments, which statement is correct?

A. Required for all spectroscopic studies

B. Cell or cuvette made of material that is transparent to radiation in the spectral region of interest

C. Plastic containers cannot be used for any spectrum

D. Crystalline sodium chloride for cell windows in UV

 

7. Which statement for photon is wrong?

A. Particles with electromagnetic radiation as a stream of discrete particles or wave packets of energy

B. Energy is proportional to radiation frequency

C.Possess wave-particle duality

D. Behavior is completely explained by classical mechanics

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