1.Write the mathematical expression for Beer's law and explain each symbol in the expression.2.Use Figure 7.2 to estimate the approximate wavelength of maximum absorption for an aque-ous solution of each of the following:Hemoglobin (the molecule that gives blood its color)a.nmChlorophyll (the pigment that gives algae their color)b.nmFill in the following for the correspondence between % transmittance and absorbance:3.1.00 absorbanceabsorbance100.0 % T1% T0.50 absorbance =absorbance50.0 % T = 4. A solution has 21.7% transmittance in a cell with a path length of 1.00 cm. If the materialresponsible for the absorption of light has a molar absorptivity e = 38.7 (L/mole-cm) at thewavelength used, calculate the concentration of this solution in mole/L. (Show all work.)

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Principles of Modern Chemistry

8th Edition

ISBN:9781305079113

Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Chapter20: Molecular Spectroscopy And Photochemistry

Section: Chapter Questions

Problem 5P

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Caffeine is a common organic molecule found in many beverages such as coffee, tea,and cola. It is a stimulant to the central nervous system. That is why many students drink coffee or soda to help them feel alert.Like many conjugated organic molecules, caffeine absorbs radiation with a wavelength around 260 nm.In this analysis, the class of Ms Judy determined the caffeinecontentin Red Bull energy drinkusing UV-vis spectroscopy a. .Construct a calibration curve. b.What is the concentration of caffeine in the unknown sample? c. Complete the table
Why must solutions with high concentrations be diluted prior to analysis via Beer's Law? A. The relationship between absorbance and concentration is not linear at high concentrations. B. The detector will reach its detection threshold. C. The photon source is too weak to provide accurate results. D. There is no need to work with dilute concentrations; any concentrations will work. E. The molar absorptivity of a compound is dependent on its concentration.
Using beer lambert's law: A= e.c.l ( l is the path length=1cm) determine the extinciton coeffiecient from the absorbance vs concentration graph.
You determine the Mg concentration of a water sample using atomic absorption spectroscopy (AAS). The sample is first diluted: 4.00 mL of the water sample is diluted to a final volume of 25.0 mL. The AAS reading for the diluted sample is 1.755 ppm. What was the Mg concentration of the original water sample, in ppm? (Enter the numerical value without units.)
From the following options what's the reason why we use absorption and not percent transmittance for UV-Vis spectrophotometry? The percent transmittance is not detectable The absorption has better resolution The food coloring reacts to the light if using the percent transmittance The absorption is directly proportional to the concentration of the solution None of the above
3. In a fluorescence spectrum, what quantities are plotted on the x- and y-axes? In a calibration curve for a fluorescence experiment, what quantities are plotted on the x- and y-axes? 4. Explain the difference between an excitation spectrum and an emission spectrum. Why does the excitation spectrum occur at lower wavelengths than the emission spectrum? 5. What is the main difference in the spectra resulting from atomic emission and molecular emission? 6. Describe how you would use serial dilution to prepare 100 mL of a 0.50 ppm QS solution from a 10 ppm stock QS solution.
A student measures the absorbence of an unknown after placing a sample in a cuvette which contained some water. How will this affect the concentration he reports?
a student in the previous question measured the absorbance of green light from a beverage that contains Red#40 and She finds that the absorbance of the beverage is 0.322. Using the calibration curve from the previous question, what would be the concentration (mM) of Red#40 in the beverage? 0.0322, 0.0497, 2.069, or 0.0008
1. Several procedures for the analysis of caffeine involve extracting the caffeine into an organic non-polar solvent (such as methylene chloride CH2Cl2). This about your experiment and what is discussed in the introduction. What might the advantage be for this approach? What is a disadvantage? 2. Think about what you said in the question above. If your sample was espresso, would your answer change? Why? 3. I was careless and ended up using a wavelength other than the peak (Say 250 nm). Would I still be able to carry out the experiment successfully? What might be the reason that I do not get optimum results? 4. I carried out the experiment using a scratched (or several scratches) cuvet for one standard, and clear cuvets for others. How might my calibration curve change (improve or get worse and why)? Pllllz answer these questions as soon as possible
What do you understand by estimation? Justify that using higher values of Cl gives better estimations.
Which mathematical relationship is most useful to relate concentration to the amount of light passing through the solution? (% Transmission, or Absorbance), Why?
Carbon monoxide is another common poison found in the forensic laboratory. Explain how UV-visible spectrophotometry is applied to quantify carbon monoxide in blood samples.

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