1.     You are having problems getting good transfection efficiency when you put your plasmid in e. coli. You want to freeze down a stock of e. coli that is more than 99% transfected. What modifications can you make to your plasmid and what steps can you take to ensure that 99% of your e. coli are transfected?

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter17: Bacterial And Viral Genetics
Section: Chapter Questions
Problem 3TYK: Which of the following is not correct for bacterial conjugation? a. Both Hfr and F+ bacteria have...
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1.     You are having problems getting good transfection efficiency when you put your plasmid in e. coli. You want to freeze down a stock of e. coli that is more than 99% transfected. What modifications can you make to your plasmid and what steps can you take to ensure that 99% of your e. coli are transfected? 

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