Question
Asked Sep 2, 2019
108 views

1. You dilute 1 mL of bacterial culture with 99 mL of water. What is the resulting dilution factor?  

2. What purposes does heat-fixing a slide serve?

3. ______ stains are attracted to the negatively charged surface of bacterial cells. 

 

check_circle

Expert Answer

Step 1

1. Dilution factor:

In order to calculate the Dilution factor, the final volume is to be divided by the initial volume.

 

help_outline

Image Transcriptionclose

DF Vf Vi where DF Dilution Factor Vf Final Volume = Aliquot Volume Diluent Volume Vi Initial Aliquot Volume

fullscreen
Step 2

Therefore, the dilution factor of 1 mL of the bacterial culture dissolved in 99 mL of water is as follows:

help_outline

Image Transcriptionclose

DF Vf/ Vi (199) mL 1 mL = 100mL 1 mL DF 100 mL

fullscreen
Step 3

2. Purpose of heat-fixing a slide:

Heat fixing helps to denature the bacterial enzymes, preventing them from digesting cell parts, which otherwise causes the cells to break by a process known as autolysis. The heat also enhances the adherence of bacterial ce...

Want to see the full answer?

See Solution

Check out a sample Q&A here.

Want to see this answer and more?

Solutions are written by subject experts who are available 24/7. Questions are typically answered within 1 hour.*

See Solution
*Response times may vary by subject and question.
Tagged in

Science

Biology

Microbiology

Related Biology Q&A

Find answers to questions asked by student like you
Show more Q&A
add
question_answer

Q: What is environmental microbiology?

A: Microbiology is an important branch of science that deals with microbes. It is the study of microsco...

question_answer

Q: How are tissues used in the body to create specific body compartments and why is it necessary?

A: Tissues are an aggregation of cells and other extracellular substances which perform a specific task...

question_answer

Q: uestions What is the purpose of the soft agar? 1

A: The questions ask about the role of soft agar.

question_answer

Q: 5a. Define each of the 3 categories of mammals: Eutherians Marsupials Monotremes:

A: Mammals belong to the class Mammalia. They are the warm-blooded animals and rule the terrestrial eco...

question_answer

Q: What are the factors that influence the productivity of tropical forests?

A: The questions ask about the factors affecting the productivity of tropical forests.

question_answer

Q: Propose an experiment and suggest a control treatment for each of the following questions.  Do beard...

A: Sex reversal may take place in bearded dragon if the eggs are incubated at 32°C or above. In bearded...

question_answer

Q: How does bottleneck effect relate to the founder effect? is the founder effect a consequence of the ...

A: Genetic drift, also known as Sewall Wright effect or allelic drift, is the change over time of the r...

question_answer

Q: i need answer for this questions?   Anatomical position Directional terms (superior/inferior, medial...

A: Hello there! Since you have posted multiple questions, I will be answering the first 3 questions. If...

question_answer

Q: Define the keywords: Dominate, Recessive, Heterozygous, Homozygous Then create two ven-diagram: Domi...

A: A genotype is referred to as the genetic makeup of an individual. A pair of different version of a g...