1.Describe the difference between the appearance of surface and subsurface colonies in a pour plate. If this is the same bacterial species, why do these differences in colonial growth occur? 2.Define the term "colony" as it relates to bacterial growth on solid media

1 View and DRAW observations of prepared slides: Bacteria Special Stains a. Endospore Stain: Clostridium tetani and Clostridium botulinum-Label: endospores, note location of endospore for each species b.) Flagella Stain: Proteus vulgaris and Spirillum-MY- volutans-Label: flagella, identify flagella arrangement on each type of organism. P

5. Using a pencil as your loop draw the streaking pattern used on a Petri plate to best achieve isolated colonies. How many times do you dip your loop into a broth culture? Number each streak in order.

14. Discuss the significant similarities and differences between Gram-negative and Gram-positive bacteria. Be as thorough and as detailed as possible (compare their width, spacing, vertical and horizontal lattice composition etc). Diagrams are great.

A. Antibiotics, such as gentamicin, are used in media to prevent growth of susceptible microbes. Is gentamicin media a selective, differential or enrichment media? B. Sorbitol is a sugar alcohol that all Escherichia bacteria can normally ferment into an acidic waste compound. This acidic end product is detected by including a pH indicator in the agar and noticing a color change. The strain of Escherichia that is a common food pathogen, E. coli O157:H7, however can not ferment sorbitol. It will grow similar to other E. coli, but not change the agar color. Is sorbitol in nutrient agar a selective, differential or enrichment media?​

1. What is the essence of the II stage of isolation of a pure culture of aerobic and anaerobic microorganisms? How is the purity of the selected culture evaluated? What are the characteristics of the growth of microorganisms on liquid nutrient media?