10. Compare and contrast bacterial conjugation, transformation, and transduction by completing the matching exercise below: 1. Conjugation 2.Transformation 3. Transduction 4. F* strain 5. F' strain 6. Auxotroph 7. Prototroph 8. Ht strain
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- Which of the following is not correct for bacterial conjugation? a. Both Hfr and F+ bacteria have the ability to code for a sex pilus. b. After an F- cell has conjugated with an F+, its plasmid holds the F+ factor. c. The recipient cell is Hfr following conjugation. d. In an HfrF- conjugation, DNA of the main chromosomemoves to a recipient cell. e. Genes on the F factor encode proteins of the sex pilus.1. What is the importance of using freshly prepared KOH in testing the bacteria? Explain this comprehensively and please, do not just copy from somewhere.1) Which of the following apply(ies) to Bacillus cereus and Bacillus anthracis? Choose all that apply: a) B. anthracis forms spores while B. cereus does not b) B. cereus is more lethal when it causes an infection c) B. anthracis has extra-chromosomal DNA that makes it more pathogenic d) Despite their similar names, the two are actually not at all related e) The 16S rRNA gene sequences of these bacteria are more than 99% identical, meaning that they are actually members of the same species
- The term "Superbug" refers to? A. Certain bacteria strain that can grow in all kinds of unfavorable conditiions B. Certain bacteria strain is more toxic than all the viruses C. Certain bacteria strain that is resistant to all available antibiotics D. Certain bacteria strain that grows faster than any other strains1. Write if the statement is true and modify the statement with the correct answer if its false a.Blue colonies in the white-blue screening provide sufficient information on the transfer of recombinant DNA in bacterial cells. b.In blue-white screening, it is only the blue colonies that have survived the effect of antibiotics in the media. c.Bacterial transformation simply means the insertion of a recombinant plasmid into a bacterial cell. d.Competent cells are those bacterial cells that can accept the entry or re-entry of plasmids.Give 5 advantages and disadvantages of GMOs ( genetically modified organism ).
- 2. Explain how the overuse of antibiotics promotes resistance in a population of bacteria.The likelihood of co-transformation of two bacterial genes increases: a. As their distance apart on the chromosome increases b. As their distance apart on the chromosome decreases c. As the transducing phage enter the lytic cycle d. As recombination rate decreases e. None of these is correc1- which of the following can be used for treating/combating bacterial infections a. targeting spikes or attachment molecules b. phages c. reverse transcriptase inhibitors d. agent that turns ON gene expression e. agents that promote phage conversion 2- can DNA replication result in genetic change? explain asap please
- 1. What extra step need to be taken after collecting samples of many viruses before PCR can be performed? What enzyme will accomplish the extra step?Describe the 3 ways in which bacteria can transfer genetic material to each other via horizontal gene transfer. Be sure to explain the following: How is DNA transferred between bacteria, is there direct contact between bacteria and whether a plasmid is involved or not.The addition of DNase to a mixture of donor and recipientcells will prevent gene transfer viaa) DNA transformation.b) chromosome transfer by conjugation.c) plasmid transfer by conjugation.d) generalized transduction.