12. Suppose an enzyme is known to obey Michaelis-Menten kinetics, but the parameters V and K are not known. Since two parameter values are missing, we might be able to find them by two independent measurements. So, suppose that two data points (81, R1) = (2 µM, 0.5 µM/s) and (82, R2) = (5 µM, 1 µM/s) are measured. By substituting the data points into the Michaelis-Menten law, find values of K and V that fit the data.

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12. Suppose an enzyme is known to obey Michaelis-Menten kinetics, but the parameters V and
K are not known. Since two parameter values are missing, we might be able to find them by
two independent measurements. So, suppose that two data points
($1, R1) = (2 µM, 0.5 µM/s)
and
(s2, R2) = (5 µM, 1 µM/s)
are measured. By substituting the data points into the Michaelis-Menten law, find values of
K and V that fit the data.
13. Graph the production rate function R(s) for the parameter values found in the previous step.
Also plot the two data points. How well does the curve fit the two data points?
Transcribed Image Text:12. Suppose an enzyme is known to obey Michaelis-Menten kinetics, but the parameters V and K are not known. Since two parameter values are missing, we might be able to find them by two independent measurements. So, suppose that two data points ($1, R1) = (2 µM, 0.5 µM/s) and (s2, R2) = (5 µM, 1 µM/s) are measured. By substituting the data points into the Michaelis-Menten law, find values of K and V that fit the data. 13. Graph the production rate function R(s) for the parameter values found in the previous step. Also plot the two data points. How well does the curve fit the two data points?
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